Tested Applications
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26935-1-AP targets Fibrillin 1 in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25342 Product name: Recombinant human FBN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 783-850 aa of BC146854 Sequence: NTPGSFVCTCPKGFIYKPDLKTCEDIDECESSPCINGVCKNSPGSFICECSSESTLDPTKTICIETIK Predict reactive species |
| Full Name | fibrillin 1 |
| GenBank Accession Number | BC146854 |
| Gene Symbol | Fibrillin 1 |
| Gene ID (NCBI) | 2200 |
| RRID | AB_2880690 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35555 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome. This antibody is specific to Fibrillin-1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Fibrillin 1 antibody 26935-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Fibrillin 1 Polyclonal antibody (Cat# 26935-1-AP) has a total of 2 citations. These publications appear in Frontiers in Immunology and Matrix Biology Plus. The cited research spans two fields: one study investigates cancer-associated fibroblasts and their prognostic value in high-grade serous ovarian carcinoma, while the other examines extracellular matrix remodeling and type III collagen enrichment in calcific aortic valve disease. Both citations used the antibody for IHC applications in human samples.
| Species | Application | Title |
|---|---|---|
Front Immunol Deciphering the immune landscape dominated by cancer-associated fibroblasts to investigate their potential in indicating prognosis and guiding therapeutic regimens in high grade serous ovarian carcinoma | ||
Matrix Biol Plus Extracellular matrix remodeling in calcific aortic valve disease: Localized enrichment of type III collagen and LTBP-4. |
Reviews
What customers say
One reviewer rated this product 4 out of 5 stars, using it for immunofluorescence at a 1:100 dilution. They reported that it showed the expected signal with minimal background, indicating good specificity and performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ramona (Verified Customer) (08-09-2023) | Showed the expected signal, with not so much background!
|
