Fibrillin 1 Polyclonal antibody

Fibrillin 1 Polyclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 26935-1-AP

Synonyms

FBN, FBN1, fibrillin 1, MASS, MFS1, OCTD, SGS, WMS

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Validation Data Gallery

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  IHC staining of human breast cancer using 26935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26935-1-AP (Fibrillin 1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26935-1-AP (Fibrillin 1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 26935-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 26935-1-AP (Fibrillin 1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A431 using 26935-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Fibrillin 1 (26935-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells
• Positive FC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

26935-1-AP targets Fibrillin 1 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibrillin 1 fusion protein Ag25342
• Full Name: fibrillin 1
• GenBank Accession Number: BC146854
• Gene Symbol: FBN1
• Gene ID (NCBI): 2200
• RRID: AB_2880690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome. This antibody is specific to Fibrillin-1.

Publications

Species	Application	Title
human	IHC	Front Immunol; Deciphering the immune landscape dominated by cancer-associated fibroblasts to investigate their potential in indicating prognosis and guiding therapeutic regimens in high grade serous ovarian carcinoma; Authors - Yimin Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ramona (Verified Customer) (08-09-2023)

Showed the expected signal, with not so much background!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100