Fibrillin 1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of HeLa using 83836-4-RR (same clone as 83836-4-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 83836-4-RR (Fibrillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83836-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 83836-4-RR (same clone as 83836-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83836-4-RR (Fibrillin 1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83836-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83836-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83836-4-RR against Human Fibrillin 1 were performed. The affinity constant is 51.3 nM.

Fibrillin 1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83836-4-PBS

Clone No.240960G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

FBN1, 240960G12, Asprosin, FBN, Fibrillin-1

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Product Information

83836-4-PBS targets Fibrillin 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30283

  Product name: Recombinant human FBN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 367-462 aa of BC146854

  Sequence: SPGVTVAPEMCPIRATEDFNKLCSVPMVIPGRPEYPPPPLGPIPPVLPVPPGFPPGPQIPVPRPPVEYLYPSREPPRVLPVNVTDYCQLVRYLCQN

• Full Name: fibrillin 1
• Observed Molecular Weight: 350 kDa
• GenBank Accession Number: BC146854
• Gene Symbol: Fibrillin 1
• Gene ID (NCBI): 2200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P35555
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome.