Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20645-1-AP targets Fibrinogen Alpha Chain in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9567 Product name: Recombinant human FGA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-218 aa of BC020764 Sequence: ADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS Predict reactive species |
| Full Name | fibrinogen alpha chain |
| Calculated Molecular Weight | 866 aa, 95 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC020764 |
| Gene Symbol | Fibrinogen Alpha Chain |
| Gene ID (NCBI) | 2243 |
| RRID | AB_2878715 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Fibrinogen Alpha Chain antibody 20645-1-AP | Download protocol |
| WB protocol for Fibrinogen Alpha Chain antibody 20645-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Fibrinogen Alpha Chain Polyclonal antibody (Cat# 20645-1-AP) has 25 citations. These appear in journals including Nature, Nature Communications, ACS Nano, Cardiovascular Diabetology, Genes & Diseases, Advanced Science, Phytomedicine, Free Radical Biology and Medicine, Neurotherapeutics, Frontiers in Immunology, Journal of Thrombosis and Haemostasis, and Frontiers in Neuroscience. Research fields span hepatocellular carcinoma, sepsis and lung injury, wound healing, thrombosis and coagulation, stroke, angiogenesis, renal cell carcinoma, and liver regeneration.
| Species | Application | Title |
|---|---|---|
Exploration (Beijing) Targeting eEF1A1 With Baicalein to Block eEF1A1/Prdx4 Interaction for Treating Sepsis-Mediated Lung Injury | ||
Nat Commun Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies. | ||
Nat Commun Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarring | ||
ACS Nano Nanoscaled Titanium Oxide Layer Provokes Quick Osseointegration on 3D-Printed Dental Implants: A Domino Effect Induced by Hydrophilic Surface |
Reviews
What customers say
Users report strong performance for this Fibrinogen Alpha Chain antibody in both immunofluorescence and immunohistochemistry. One reviewer used it at 1/200 dilution on PFA-fixed mouse brain cryosections with overnight incubation at 4°C, achieving clear staining in stroke tissue. Another confirmed reliable fibrinogen labeling in choroid plexus samples and noted the antibody retained full activity after being stored at −20°C for over a year without aliquoting. Overall, reviewers highlight consistent staining quality and excellent storage stability.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marion (Verified Customer) (07-15-2025) | Immunohistofluorescence (PFA-fixed cryosections) image of Fibrinogen Alpha Chain stained mouse stroke brain. Blocking step : 10 % Donkey Serum + 1% BSA + 0.02% Triton as blocking agent for 2 hours at RT Primary antiboby incubation time : 1 night / 4°C Secondary antibody : AlexaFluor 568 (ab175470 ; Abcam) ; 1/200
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Lillian (Verified Customer) (05-27-2025) | Labeled fibrinogen in our tissue samples. Kept at -20C for the past year and worked even when not aliquoted.
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