Fibrinogen Alpha Chain Polyclonal antibody

• Featured Product
• KD/KO Validated

Fibrinogen Alpha Chain Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 20645-1-AP

Synonyms

FGA, Fib2, fibrinogen alpha chain

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Validation Data Gallery

• 

  WB analysis of mouse liver using 20645-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20645-1-AP (Fibrinogen alpha chain antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20645-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20645-1-AP (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human colon tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

20645-1-AP targets Fibrinogen Alpha Chain in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibrinogen Alpha Chain fusion protein Ag9567
• Full Name: fibrinogen alpha chain
• Calculated Molecular Weight: 866 aa, 95 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC020764
• Gene Symbol: Fibrinogen Alpha Chain
• Gene ID (NCBI): 2243
• RRID: AB_2878715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies.; Authors - Di-Wei Zheng
human	WB,IP	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma
human	WB	Aging (Albany NY); Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients.; Authors - Wenjing Wang
mouse	WB	J Cell Mol Med; Over-expression of Dyrk1A affects bleeding by modulating plasma fibronectin and fibrinogen level in mice; Authors - Guillaume Postic
mouse		J Thromb Haemost; Chronic liver injury drives non-traditional intrahepatic fibrin(ogen) cross-linking via tissue transglutaminase.; Authors - L G Poole
human	IF	Phytomedicine; Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.; Authors - Yan Wang