|Positive WB detected in||A375 cells, A549 cells, HEK-293 cells, HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
28208-1-AP targets Flotillin 2 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Flotillin 2 fusion protein Ag28308|
|Full Name||flotillin 2|
|Calculated molecular weight||379 aa, 42 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC017292|
|Gene ID (NCBI)||2319|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
Cancer Commun (Lond)
Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer
J Exp Clin Cancer Res
RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization
Int J Biol Sci
miR-34a-5p functions as a tumor suppressor in head and neck squamous cell cancer progression by targeting Flotillin-2.
Int J Biol Sci
TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma.
PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells.
Amyloid futures in the expanding pathology of brain aging and dementia
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guorong (Verified Customer) (03-22-2022)
Very weak bands with strong background were detected at 48 kDa