Flow Cytometry Perm Buffer (10X)

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  1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed with 1X Transcription Factor Fix/Perm buffer (PF00011-A) and permeabilized with 1X Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).
  

Cat No. PF00011-C

Concentration:

10x

Applications:

FC

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Product Information

Flow Cytometry Perm Buffer (10X) Provided as a concentrate which, when diluted with distilled water to a 1X solution, provide best results during intracellular staining of cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and washing steps.

Storage

4°C

Expiration Date

12 months after receiving.

Cited in Article as

PF00011-C, Flow Cytometry Perm Buffer (10X), Proteintech, IL, USA