Fluorescein (FITC)–conjugated Affinipure Goat Anti-Rat IgG(H+L)

Applications

IF, Flow cyt

Conjugate

FITC

Host

Goat

Reactivity

Rat

Cat no : SA00003-11

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Validation Data Gallery

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  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107b (65052-1-Ig, Clone:ABL-93) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) (SA00003-11) at dilution 1:50 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH.

Product Information

• Applications: IF, Flow cyt
• Host Species: Goat
• Species Reactivity: Rat
• Physical State: Liquid
• Suggested Dilution Range: 1:20-1:100 for most applications
• Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Storage Buffer: 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6
• Preservative: 0.02% Sodium Azide
• Storage Instructions: Store at -20°C. Avoid repeated freeze / thaw cycles.
• Fluorophore: Fluorescein-5-isothiocyanate(FITC) Amax=492nm;Emax=520nm
• Fluorophore Protein: 8.8ug/mg; 3.3 moles FITC per mole IgG
• Stabilizer: 5mg/ml BSA
• Concentration: 0.2mg/mL
• RRID: AB_2890989

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