Fluorescein (FITC)–conjugated Goat Anti-Rat IgG(H+L)

Applications

IF, FC

Conjugate

FITC

Host

Goat

Reactivity

Rat

Publications

62

Cat no : SA00003-11

---

Validation Data Gallery

• 

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107b (65052-1-Ig, Clone:ABL-93) and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) (SA00003-11) at dilution 1:50 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH.

Product Information

• Concentration: 0.6 mg/mL
• Applications: IF, FC
• Host Species: Goat
• Species Reactivity: Rat
• Physical State: Liquid
• Suggested Dilution Range: 1:20-1:100 for most applications
• Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Storage Buffer: 0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.02% Sodium Azide, pH 7.6.
• Preservative: 0.02% Sodium Azide
• Storage Instructions: Store at -20°C. Stable for one year after shipment.
• Fluorophore: Fluorescein-5-isothiocyanate (FITC) Amax=492nm;Emax=520nm
• Fluorophore Protein: 8.8ug/mg; 3.3 moles FITC per mole IgG
• Stabilizer: 5 mg/ml BSA
• Clonality: Polyclonal
• RRID: AB_2890989

Publications

Title
Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv
Nat Commun; Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells.; Authors - Ruifeng Zhao
ACS Appl Mater Interfaces; Matrix Stiffness of GelMA Hydrogels Regulates Lymphatic Endothelial Cells toward Enhanced Lymphangiogenesis; Authors - Ziyue Qin
Int J Biol Sci; NLRP3 Regulates Mandibular Healing through Interaction with UCHL5 in MSCs; Authors - Wenhua Zhao
ACS APPL MATER INTER; Self-promoted Albumin-Based Nanoparticles for Combination Therapy against Metastatic Breast Cancer via a HyperthermiaInduced “Platelet Bridge”; Authors - Wei Zhao
Clin Immunol; Donor graft METTL3 gene transfer ameliorates rat liver transplantation ischemia-reperfusion injury by enhancing HO-1 expression in an m6A-dependent manner; Authors - Song Xiang

---