Frizzled 10 Recombinant monoclonal antibody

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• Frizzled 10 Recombinant monoclonal antibody (85829-2-RR)

• 

  WB analysis using 85829-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85829-2-RR (Frizzled 10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 85829-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Frizzled 10 antibody (85829-2-RR, Clone: 243131B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity of 85829-2-RR

  Biolayer interferometry (BLI) kinetic assays of 85829-2-RR against Human Frizzled 10 were performed. The affinity constant is 5.91 nM.

Frizzled 10 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85829-2-RR

Clone No.243131B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FZD10, CD350, Frizzled-10, FZ 10, Fz-10

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85829-2-RR targets Frizzled 10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12867

  Product name: Recombinant human FZD10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 23-229 aa of BC074998

  Sequence: SMDMERPGDGKCQPIEIPMCKDIGYNMTRMPNLMGHENQREAAIQLHEFAPLVEYGCHGHLRFFLCSLYAPMCTEQVSTPIPACRVMCEQARLKCSPIMEQFNFKWPDSLDCRKLPNKNDPNYLCMEAPNNGSDEPTRGSGLFPPLFRPQRPHSAQEHPLKDGGPGRGGCDNPGKFHHVEKSASCAPLCTPGVDVYWSREDKRFAVV

• Full Name: frizzled homolog 10 (Drosophila)
• Calculated Molecular Weight: 581 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC074998
• Gene Symbol: Frizzled 10
• Gene ID (NCBI): 11211
• RRID: AB_3744405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULW2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FZD10 (Frizzled homolog 10) is a member of the frizzled gene family, and considered as a seven-transmembrane Wnt-signaling receptor. FZD10 is expressed at high levels on the cell surface of almost all synovial sarcoma tissues but absent in most normal organs except for placenta. Up-regulation of FZD10 mRNA in several types of human cells might lead to carcinog