GAA Polyclonal antibody

GAA Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14367-1-AP

Synonyms

Acid maltase, Aglucosidase alfa, GAA, glucosidase, alpha; acid, LYAG, Lysosomal alpha glucosidase



Tested Applications

Positive WB detected inDU 145 cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue, mouse skeletal muscle tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14367-1-AP targets GAA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAA fusion protein Ag5690
Full Name glucosidase, alpha; acid
Calculated Molecular Weight 105 kDa
Observed Molecular Weight70-76 kDa
GenBank Accession NumberBC040431
Gene Symbol GAA
Gene ID (NCBI) 2548
RRIDAB_2107667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAA(Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal a-glucosidase, and both N-terminal and C-terminal processing occur. The full sequence can be further processed into a mature form. The 105 kDa GAA can be cleaved into 70 kDa or 76 kDa form.

Protocols

Product Specific Protocols
WB protocol for GAA antibody 14367-1-APDownload protocol
IHC protocol for GAA antibody 14367-1-APDownload protocol
IP protocol for GAA antibody 14367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis

Authors - Lindsey R Conroy
humanIF

J Med Chem

5-C-Branched Deoxynojirimycin: Strategy for Designing a 1-Deoxynojirimycin-Based Pharmacological Chaperone with a Nanomolar Affinity for Pompe Disease.

Authors - Atsushi Kato
humanIF

Org Biomol Chem

Introduction of C-alkyl branches to L-iminosugars changes their active site binding orientation

Authors - Atsushi Kato
zebrafishWB

DNA Cell Biol

Disruption of the gaa Gene in Zebrafish Fails to Generate the Phenotype of Classical Pompe Disease.

Authors - Jing Wu
IF

J Med Chem

Design and Pharmacological Chaperone Effects of N-(4'-Phenylbutyl)-DAB Derivatives Targeting the Lipophilic Pocket of Lysosomal Acid α-Glucosidase

Authors - Atsushi Kato
mouse,ratWB,IHC

Phytother Res

D-pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin-mediated endoplasmic reticulum stress and glycophagy signaling pathway

Authors - Xiaoli Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

CHRISTINE (Verified Customer) (09-27-2019)

will continue to order here in the future.

  • Applications: Western Blot, Flow Cytometry, Cell culture,
  • Cell Tissue Type: IgG