GAA Polyclonal antibody, PBS Only

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• GAA Polyclonal antibody, PBS Only (29993-1-PBS)

• 

  WB analysis using 29993-1-AP (same clone as 29993-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29993-1-AP (GAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 29993-1-AP (same clone as 29993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29993-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 29993-1-AP (same clone as 29993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29993-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29993-1-PBS in a different storage buffer formulation.

GAA Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29993-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

29993-1-PBS targets GAA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28970

  Product name: Recombinant human GAA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 38-230 aa of BC040431

  Sequence: VPRELSGSSPVLEETHPAHQQGASRPGPRDAQAHPGRPRAVPTQCDVPPNSRFDCAPDKAITQEQCEARGCCYIPAKQGLQGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTATLTRTTPTFFPKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPHVHSRAPSPLYSVEFSEEPFGVIVRRQLDGRV

• Full Name: glucosidase, alpha; acid
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC040431
• Gene Symbol: GAA
• Gene ID (NCBI): 2548
• RRID: AB_2923627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10253
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GAA (Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal α-glucosidase, and both N-terminal and C-terminal processing occur. Typically, GAA is synthesized as an immature glycoprotein precursor (110 kDa) in the endoplasmic reticulum and undergoes a seriesof proteolytic and N-glycan processing events to yield an intermediate (95 kDa) and two lysosomal (76 and 70 kDa) isoforms. This antibody can detect the 110 kDa precursor form.