GABARAP Polyclonal antibody

GABARAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 22439-1-AP

Synonyms

MM46, HT004, Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, FLC3B

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Validation Data Gallery

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  WB analysis using 22439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22439-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 22439-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22439-1-AP

  Chloroquine treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 22439-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22439-1-AP (GABARAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, SH-SY5Y cells, Chloroquine treated HEK-293 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22439-1-AP targets GABARAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GABARAP fusion protein Ag18209
• Full Name: GABA(A) receptor-associated protein
• Calculated Molecular Weight: 117 aa, 14 kDa
• Observed Molecular Weight: 19-21 kDa
• GenBank Accession Number: BC106749
• Gene Symbol: GABARAP
• Gene ID (NCBI): 11337
• RRID: AB_2879106
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other