GABARAP Recombinant monoclonal antibody

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• GABARAP Recombinant monoclonal antibody (86165-3-RR)

• 

  WB analysis using 86165-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86165-3-RR (GABARAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86165-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using GABARAP antibody (86165-3-RR, Clone: 250354C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GABARAP Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86165-3-RR

Clone No.250354C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FLC3B, GABA(A) receptor-associated protein, Gamma-aminobutyric acid receptor-associated protein, HT004, MM46

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, SH-SY5Y cells, RAW 264.7cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: Chloroquine treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86165-3-RR targets GABARAP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GABA(A) receptor-associated protein
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14-18 kDa
• GenBank Accession Number: BC106748
• Gene Symbol: GABARAP
• Gene ID (NCBI): 11337
• RRID: AB_3744675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95166
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.