GABA B receptor 1 Polyclonal antibody

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• GABA B receptor 1 Polyclonal antibody (13940-1-AP)

GABA B receptor 1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13940-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

GABAB receptor 1, GABBR1, GABA B R1, GABA BR1, GABA-B receptor 1

• 

  WB analysis using 13940-1-AP

  Boiled and 37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 13940-1-AP (GABA B receptor 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 13940-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13940-1-AP (GABA B receptor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13940-1-AP (GABA B receptor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, 37°C incubated mouse brain tissue, rat brain tissue, 37°C incubated rat brain tissue
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13940-1-AP targets GABA B receptor 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4838

  Product name: Recombinant human GABBR1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 101-471 aa of BC041332

  Sequence: SKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAARMWNLIVLSYGSSSPALSNRQRFPTFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEVFTSTLDDLEERVKEAGIEITFRQSFFSDPAVPVKNLKRQDARIIVGLFYETEARKVFCEVYKERLFGKKYVWFLIGWYADNWFKIYDPSINCTVDEMTEAVEGHITTEIVMLNPANTRSISNMTSQEFVEKLTKRLKRHPEETGGFQEAPLAYDAIWALALALNKTSGGGGRSGVRLEDFNYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWTLIEQLQGGSYKKIGYYDSTKDDLSWSKTDKWIGGSPPADQTLVIKTFRFLS

• Full Name: gamma-aminobutyric acid (GABA) B receptor, 1
• Calculated Molecular Weight: 961 aa, 108 kDa
• Observed Molecular Weight: 105-130 kDa
• GenBank Accession Number: BC041332
• Gene Symbol: GABBR1
• Gene ID (NCBI): 2550
• RRID: AB_2108159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBS5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABA B receptor 1 (GABBR1) is an essential protein subunit that forms the functional GABA B receptor, a metabotropic receptor for the primary inhibitory neurotransmitter in the central nervous system, gamma-aminobutyric acid (GABA). Unlike the fast-acting ionotropic GABA A receptors, GABA B receptors mediate slow, prolonged inhibitory signaling through G-proteins. Two bands with the apparent molecular weights of 130 and 105 kDa were corresponding to GABAB1a and GABAB1b proteins, were identified in the brain extract and membrane extract of wild-type mice (PMID: 12970075).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Biochem Biophys Res Commun; AJAP1 affects behavioral changes and GABABR1 level in epileptic mice.; Authors - Mingli Zhang
mouse	WB	J Hazard Mater; Cu(OH)(2) nanopesticide induced adolescent social behavior deficits via long noncoding RNA-mediated synaptic network dysfunction.; Authors - Zhihua Ren
mouse	WB	Brain Behav Immun; Targeted fecal microbiota transplantation ameliorates autism-like behaviors via gut-brain axis and excitatory/inhibitory balance restoration in a propionic acid mouse model.; Authors - Jianquan He