Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27567-1-AP targets GABBR2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25886 Product name: Recombinant human GABBR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 685-883 aa of BC035071 Sequence: KLITLRTNPDAATQNRRFQFTQNQKKEDSKTSTSVTSVNQASTSRLEGLQSENHHLRMKITELDKDLEEVTMQLQDTPEKTTYIKQNHYQELNDILNLGNFTESTDGGKAILKNHLDQNPQLQWNTTEPSRTCKDPIEDINSPEHIQRRLSLQLPILHHAYLPSIGGVDASCVSPCVSPTASPRHRHVPPSFRVMVSGL Predict reactive species |
| Full Name | gamma-aminobutyric acid (GABA) B receptor, 2 |
| Calculated Molecular Weight | 941 aa, 106 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC035071 |
| Gene Symbol | GABBR2 |
| Gene ID (NCBI) | 9568 |
| RRID | AB_2880911 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75899 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GABBR2 antibody 27567-1-AP | Download protocol |
| WB protocol for GABBR2 antibody 27567-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GABBR2 Polyclonal antibody (Cat# 27567-1-AP) has 19 citations. These appear in journals including Cancer Cell, Nature Cell Biology, Nature Communications, Redox Biology, Molecular Psychiatry, Antioxidants, World Journal of Gastroenterology, Journal of Ethnopharmacology, Ecotoxicology and Environmental Safety, Biochemical Pharmacology, Life Sciences, Food & Function, Frontiers in Cellular and Infection Microbiology, Brain Research Bulletin, Neurorehabilitation and Neural Repair, Behavioural Brain Research, and Biochemical and Biophysical Research Communications. Research fields span oncology, neurology, immunology, gastroenterology, and pharmacology.
| Species | Application | Title |
|---|---|---|
Cancer Cell GABA promotes resistance to immunotherapy in patients with TLS-positive tumors. | ||
Nat Cell Biol Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression. | ||
Nat Commun Neuropeptide SP protects against colitis and linked anxiety-like behavior through the putative roles of gut microbiota and metabolite inositol. | ||
Redox Biol Activation of nuclear receptor pregnane-X-receptor protects against abdominal aortic aneurysm by inhibiting oxidative stress | ||
Mol Psychiatry Downregulation of integrin α3 in ADHD mirrored in mutant mouse model by dopamine-dependent hippocampal AMPAR expression. | ||
Antioxidants (Basel) Activation of GABABR Attenuates Intestinal Inflammation by Reducing Oxidative Stress through Modulating the TLR4/MyD88/NLRP3 Pathway and Gut Microbiota Abundance |
Reviews
What customers say
The single review for 27567-1-AP is highly positive, rated 5 stars. The customer reported successful Western Blot results using B6 mouse brain tissue, confirming the antibody's effectiveness in detecting its target. The recommended dilution was 1:1000, which produced clear positive bands. Overall, the feedback indicates reliable performance for Western Blot applications in mouse tissue, supporting the product's quality and reproducibility for researchers working with mouse brain samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
De (Verified Customer) (02-18-2020) | Got positive WB results in B6 mouse brain tissues by using this antibody.
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