GABBR2 Polyclonal antibody

GABBR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 27567-1-AP

Synonyms

G protein coupled receptor 51, GABA B R2, GABA B receptor 2, GABA BR2, GABABR2, GABBR2, Gb2, GPR51, GPRC3B, HG20, HRIHFB2099

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Validation Data Gallery

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  WB analysis using 27567-1-AP

  mouse brain tissue and rat brain tissue were subjected to SDS PAGE followed by western blot with 27567-1-AP (GABBR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 27567-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27567-1-AP (GABBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 27567-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27567-1-AP (GABBR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

27567-1-AP targets GABBR2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GABBR2 fusion protein Ag25886
• Full Name: gamma-aminobutyric acid (GABA) B receptor, 2
• Calculated Molecular Weight: 941 aa, 106 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC035071
• Gene Symbol: GABBR2
• Gene ID (NCBI): 9568
• RRID: AB_2880911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Nat Cell Biol; Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.; Authors - De Huang
human	WB	World J Gastroenterol; γ-aminobutyric acid B2 receptor: A potential therapeutic target for cholangiocarcinoma in patients with diabetes mellitus; Authors - Charupong Saengboonmee
human	WB,IHC	Biochem Biophys Res Commun; GAD1-mediated GABA elicits aggressive characteristics of human oral cancer cells; Authors - Zhichao Dou
human	IHC	Life Sci; GABA regulates the proliferation and apoptosis of head and neck squamous cell carcinoma cells by promoting the expression of CCND2 and BCL2L1; Authors - Kunliang Luo
rat	WB	Neurorehabil Neural Repair; Metabolomics-Based Effects of a Natural Product on Remyelination After Cerebral Ischemia Injury Via GABABR-pCREB-BDNF Pathway; Authors - Xiaodi Fan
mouse	WB	Behav Brain Res; Impact of Abl2/Arg Deficiency on Anxiety and Depressive Behaviors in mice; Authors - Xiaojuan Yao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

De (Verified Customer) (02-18-2020)

Got positive WB results in B6 mouse brain tissues by using this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: tissue