GABPB1 Polyclonal antibody

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• GABPB1 Polyclonal antibody (12597-1-AP)

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• KD/KO Validated

GABPB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12597-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

BABPB2, E4TF1, E4TF1 47, E4TF1 53, E4TF1B

• 

  WB analysis of mouse lung using 12597-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 12597-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 12597-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 12597-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 12597-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 12597-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 12597-1-AP

  IP result of anti-GABPB1 (IP:12597-1-AP, 4ug; Detection:12597-1-AP 1:1000) with HeLa cells lysate 520ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 12597-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GABPB1 antibody (12597-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A375 cells, MCF-7 cells, mouse lung tissue, HeLa cells, HepG2 cells, mouse testis tissue, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12597-1-AP targets GABPB1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3208

  Product name: Recombinant human GABPB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-348 aa of BC016910

  Sequence: MSLVDLGKKLLEAARAGQDDEVRILMANGAPFTTDWLGTSPLHLAAQYGHYSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHASIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELLIKYGADVHTQSKFCKTAFDISIDNGNEDLAEILQIAMQNQINTNPESPDTVTIHAATPQFIIGPGGVVNLTGLVSSENSSKATDETGVSAVQFGNSSTSVLATLAALAEASAPLSNSSETPVVATEEVVTAESVDGAIQQVVSSGGQQVITIVTDGIQLGNLHSIPTSGIGQPIIVTMPDGQQVLTVPATDIAEETVISEEPPAKRQCIEIIENRVESAEIEVRS

• Full Name: GA binding protein transcription factor, beta subunit 1
• Calculated Molecular Weight: 347 aa, 38 kDa
• Observed Molecular Weight: 36-45 kDa, 47-53 kDa
• GenBank Accession Number: BC016910
• Gene Symbol: GABPB1
• Gene ID (NCBI): 2553
• RRID: AB_10951115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q06547
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABPB1, also named as E4TF1B, GABPB, GABPB2, E4TF1-47 and E4TF1-53, is a transcription factor capable of interacting with purine rich repeats (GA repeats). It is necessary for the expression of the Adenovirus E4 gene. GABPB1 has 4 isoforms. The MW-of Beta 1 is 53kd and Beta-2 is 47kd(PMID:9525935). This antibody can recognize all the isoforms.

Publications

What published studies show

The GABP antibody (Cat# 12597-1-AP) has 12 total citations spanning journals including Cancer Cell, Nature Communications, Molecular Cell, Advanced Science, PNAS, Cell Reports, Thyroid, Scientific Reports, Brain Research Bulletin, and Biology. Research fields cover glioblastoma and TERT promoter biology, renal fibrosis in diabetic nephropathy, ferroptosis, thyroid cancer progression, melanoma apoptosis, neuronal protection, and the Hippo signaling pathway. Applications include WB, IP, ChIP, IHC, and IF in human and mouse models.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Cell; Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner.; Authors - Andrew Mancini ; KD Validated
human	ChIP	Nat Commun; INSM1 governs a neuronal progenitor state that drives glioblastoma in a human stem cell model.; Authors - Patrick A. DeSouza
mouse	WB	Nat Commun; Non-human primate seasonal transcriptome atlas reveals seasonal changes in physiology and diseases; Authors - Junfeng Chen ; KD Validated
human	IP	Mol Cell; Argininosuccinate lyase drives activation of mutant TERT promoter in glioblastomas; Authors - Zhumei Shi ; KD Validated
mouse	WB,IHC,IF	Adv Sci (Weinh); GABP Promotes Mesangial Cell Proliferation and Renal Fibrosis Through GLI1 in Diabetic Nephropathy; Authors - Lei Du
human	WB	Proc Natl Acad Sci U S A; Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage.; Authors - Alexandra M Amen