GABRA1 Polyclonal antibody

GABRA1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 12410-1-AP

Synonyms

ECA4, EJM, GABA A receptor alpha 1, GABAA receptor alpha 1, GABRA1

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Validation Data Gallery

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  WB analysis using 12410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12410-1-AP (GABRA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12410-1-AP

  IP Result of anti-GABRA1 (IP:12410-1-AP, 3ug; Detection:12410-1-AP 1:400) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of rat brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 12410-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GABRA1 antibody (12410-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 22 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

12410-1-AP targets GABRA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GABRA1 fusion protein Ag3117
• Full Name: gamma-aminobutyric acid (GABA) A receptor, alpha 1
• Calculated molecular weight: 456 aa, 52 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC030696
• Gene symbol: GABRA1
• Gene ID (NCBI): 2554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse	WB	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
human		Neurol Neuroimmunol Neuroinflamm; MRI Characteristics of Autoimmune Encephalitis With Autoantibodies to GABAA Receptor: A Case Series.; Authors - Bo Deng
mouse	WB	Mol Ther Nucleic Acids; Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.; Authors - Colleen S Stein
mouse	WB	Int J Mol Sci; The Effect of TGF-β1 Reduced Functionality on the Expression of Selected Synaptic Proteins and Electrophysiological Parameters: Implications of Changes Observed in Acute Hepatic Encephalopathy.; Authors - Mariusz Popek
mouse	WB	Cell Death Dis; Transgenic overexpression of furin increases epileptic susceptibility.; Authors - Yi Yang

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