GABRA1 Polyclonal antibody

GABRA1 Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC,ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 12410-1-AP

Synonyms

ECA4, EJM, GABA A receptor alpha 1, GABAA receptor alpha 1, GABRA1

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Validation Data Gallery

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  WB analysis using 12410-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12410-1-AP (GABRA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12410-1-AP

  IP Result of anti-GABRA1 (IP:12410-1-AP, 3ug; Detection:12410-1-AP 1:400) with mouse brain tissue lysate 3600ug.

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  FC experiment of SH-SY5Y using 12410-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GABRA1 antibody (12410-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 12 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

12410-1-AP targets GABRA1 in WB, IP, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GABRA1 fusion protein Ag3117
• Full Name: gamma-aminobutyric acid (GABA) A receptor, alpha 1
• Calculated molecular weight: 456 aa, 52 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC030696
• Gene symbol: GABRA1
• Gene ID (NCBI): 2554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
	WB	Mol Nutr Food Res; As a Histone Deacetylase Inhibitor, γ-Aminobutyric Acid Upregulates GluR2 Expression: An In Vitro and In Vivo Study.; Authors - Aiping Xing
rat	WB	Acta Biochim Biophys Sin (Shanghai); Propofol differentially induces unconsciousness and respiratory depression through distinct interactions between GABAA receptor and GABAergic neuron in corresponding nuclei.; Authors - Junli Jiang
human	WB,IF	Reprod Fertil Dev; Sperm gamma-aminobutyric acid type A receptor delta subunit (GABRD) and its interaction with purinergic P2X2 receptors in progesterone-induced acrosome reaction and male fertility.; Authors - Wenming Xu
mouse	WB	Food Funct; Ginsenoside Rg5/Rk1 ameliorated sleep via regulating the GABAergic/serotoninergic signaling pathway in a rodent model.; Authors - Jingjing Shao
mouse	WB,IP	CNS Neurosci Ther; STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons.; Authors - Juan Yang
human	WB,IF	Acta Physiol (Oxf); c-Jun N-terminal Kinase mediates prostaglandin induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression.; Authors - Renjun Wang

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