GABRA1 Polyclonal antibody

GABRA1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12410-1-AP

Synonyms

ECA4, EJM, GABA A receptor alpha 1, GABAA receptor alpha 1, GABRA1



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12410-1-AP targets GABRA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABRA1 fusion protein Ag3117
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 1
Calculated molecular weight 456 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC030696
Gene symbol GABRA1
Gene ID (NCBI) 2554
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GABRA1 antibody 12410-1-APDownload protocol
IHC protocol for GABRA1 antibody 12410-1-APDownload protocol
IP protocol for GABRA1 antibody 12410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Mol Nutr Food Res

As a Histone Deacetylase Inhibitor, γ-Aminobutyric Acid Upregulates GluR2 Expression: An In Vitro and In Vivo Study.

Authors - Aiping Xing
ratWB

Acta Biochim Biophys Sin (Shanghai)

Propofol differentially induces unconsciousness and respiratory depression through distinct interactions between GABAA receptor and GABAergic neuron in corresponding nuclei.

Authors - Junli Jiang
mouseWB

Int J Mol Sci

The Effect of TGF-β1 Reduced Functionality on the Expression of Selected Synaptic Proteins and Electrophysiological Parameters: Implications of Changes Observed in Acute Hepatic Encephalopathy.

Authors - Mariusz Popek
humanWB,IF

Reprod Fertil Dev

Sperm gamma-aminobutyric acid type A receptor delta subunit (GABRD) and its interaction with purinergic P2X2 receptors in progesterone-induced acrosome reaction and male fertility.

Authors - Wenming Xu
mouseWB

Food Funct

Ginsenoside Rg5/Rk1 ameliorated sleep via regulating the GABAergic/serotoninergic signaling pathway in a rodent model.

Authors - Jingjing Shao
mouseWB,IP

CNS Neurosci Ther

STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons.

Authors - Juan Yang