GABRA4 Polyclonal antibody, PBS Only

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• GABRA4 Polyclonal antibody, PBS Only (12979-1-PBS)

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  WB analysis of mouse skeletal muscle using 12979-1-AP (same clone as 12979-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12979-1-AP (GABRA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12979-1-PBS in a different storage buffer formulation.

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  WB analysis using 12979-1-AP (same clone as 12979-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12979-1-AP (GABRA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12979-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 12979-1-AP (same clone as 12979-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12979-1-AP (GABRA4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12979-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 12979-1-AP (same clone as 12979-1-PBS)

  IP result of anti-GABRA4 (IP:12979-1-AP, 4ug; Detection:12979-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug. This data was developed using the same antibody clone with 12979-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12979-1-AP (same clone as 12979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12979-1-AP (GABRA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12979-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12979-1-AP (same clone as 12979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12979-1-AP (GABRA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12979-1-PBS in a different storage buffer formulation.

GABRA4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12979-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

GABA A Receptor alpha 4, GABA(A) receptor subunit alpha-4, GABAA Receptor alpha 4, GABAAR subunit alpha-4, Gamma-aminobutyric acid receptor subunit alpha-4

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Product Information

12979-1-PBS targets GABRA4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3600

  Product name: Recombinant human GABRA4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 338-554 aa of BC035055

  Sequence: AAVNYFTNIQMEKAKRKTSKPPQEVPAAPVQREKHPEAPLQNTNANLNMRKRTNALVHSESDVGNRTEVGNHSSKSSTVVQESSKGTPRSYLASSPNPFSRANAAETISAARALPSASPTSIRTGYMPRKASVGSASTRHVFGSRLQRIKTTVNTIGATGKLSATPPPSAPPPSGSGTSKIDKYARILFPVTFGAFNMVYWVVYLSKDTMEKSESLM

• Full Name: gamma-aminobutyric acid (GABA) A receptor, alpha 4
• Calculated Molecular Weight: 554 aa, 62 kDa
• Observed Molecular Weight: 62-70 kDa
• GenBank Accession Number: BC035055
• Gene Symbol: GABRA4
• Gene ID (NCBI): 2557
• RRID: AB_2108996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48169
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABRA4, encoding the extrasynaptically located α4‐subunit of the GABAA receptor, is involved in the etiology of autism. (PMID: 32028044, 32033586)