GABRB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GABRB1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 20183-1-AP

Synonyms

GABA A receptor beta 1, GABA A receptor β, GABAA receptor beta 1, GABRB1

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Validation Data Gallery

• 

  WB analysis of mouse testis using 20183-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20183-1-AP (GABRB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20183-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20183-1-AP (GABRB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 20183-1-AP

  IP result of anti-GABRB1 (IP:20183-1-AP, 4ug; Detection:20183-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Product Information

20183-1-AP targets GABRB1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GABRB1 fusion protein Ag14070
• Full Name: gamma-aminobutyric acid (GABA) A receptor, beta 1
• Calculated Molecular Weight: 474 aa, 54 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC022449
• Gene Symbol: GABRB1
• Gene ID (NCBI): 2560
• RRID: AB_10638781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse	WB	iScience; Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1-/- mice; Authors - Jing Wang ; KO Validated
mouse	WB	Food Funct; Ginsenoside Rg5/Rk1 ameliorated sleep via regulating the GABAergic/serotoninergic signaling pathway in a rodent model.; Authors - Jingjing Shao
human	WB,IF	Reprod Fertil Dev; Sperm gamma-aminobutyric acid type A receptor delta subunit (GABRD) and its interaction with purinergic P2X2 receptors in progesterone-induced acrosome reaction and male fertility.; Authors - Wenming Xu
	WB	Mol Nutr Food Res; As a Histone Deacetylase Inhibitor, γ-Aminobutyric Acid Upregulates GluR2 Expression: An In Vitro and In Vivo Study.; Authors - Aiping Xing

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