GAD1 Monoclonal antibody

GAD1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2D5B7

Cat no : 67648-1-Ig

Synonyms

FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP



Tested Applications

Positive WB detected inmouse cerebellum tissue, rat brain tissue, pig brain tissue, rabbit brain tissue
Positive IHC detected inrat cerebellum tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67648-1-Ig targets GAD1 in WB, IHC,ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GAD1 fusion protein Ag21336
Full Name glutamate decarboxylase 1 (brain, 67kDa)
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC002815
Gene symbol GAD1
Gene ID (NCBI) 2571
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development(PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.

Protocols

Product Specific Protocols
WB protocol for GAD1 antibody 67648-1-IgDownload protocol
IHC protocol for GAD1 antibody 67648-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols