GAD1 Monoclonal antibody

GAD1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human, mouse, rat, pig, rabbit and More (1)







Cat no : 67648-1-Ig


FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP

Tested Applications

Positive WB detected inpig brain tissue, rat brain tissue, mouse cerebellum tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat cerebellum tissue, mouse brain tissue
Positive IHC detected inrat cerebellum tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67648-1-Ig targets GAD1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GAD1 fusion protein Ag21336
Full Name glutamate decarboxylase 1 (brain, 67kDa)
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 67 kDa, 57 kDa
GenBank Accession NumberBC002815
Gene Symbol GAD1
Gene ID (NCBI) 2571
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.


Product Specific Protocols
WB protocol for GAD1 antibody 67648-1-IgDownload protocol
IHC protocol for GAD1 antibody 67648-1-IgDownload protocol
IF protocol for GAD1 antibody 67648-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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