GADD45GIP1 Polyclonal antibody

GADD45GIP1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16260-1-AP

Synonyms

CKBBP2, CKII beta associating protein, CR6 interacting factor 1, CRIF1, GADD45GIP1, p53 responsive gene 6 protein, PLINP 1, Plinp1, PRG6

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Validation Data Gallery

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  WB analysis of mouse heart using 16260-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16260-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16260-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 16260-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16260-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16260-1-AP

  IP result of anti-GADD45GIP1 (IP:16260-1-AP, 4ug; Detection:16260-1-AP 1:400) with mouse heart tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 16260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, NIH/3T3 cells, HEK-293 cells, SKOV-3 cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16260-1-AP targets GADD45GIP1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GADD45GIP1 fusion protein Ag9299
• Full Name: growth arrest and DNA-damage-inducible, gamma interacting protein 1
• Calculated Molecular Weight: 222 aa, 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC013027
• Gene Symbol: GADD45GIP1
• Gene ID (NCBI): 90480
• RRID: AB_2108229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Science; T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration.; Authors - Isobel Okoye
human	IHC	Oncotarget; CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma.; Authors - Runzhou Zhuang
rat	WB	J Mol Med (Berl); Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.; Authors - Li Peng
mouse	WB	Burns Trauma; Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction.; Authors - Lingfei Li