GAK Polyclonal antibody

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  WB analysis of human brain using 12147-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12147-1-AP (GAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12147-1-AP (GAK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12147-1-AP (GAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12147-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12147-1-AP (GAK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GAK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12147-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.7.11.1, DNAJC26

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

12147-1-AP targets GAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2795

  Product name: Recombinant human GAK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-309 aa of BC008668

  Sequence: MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSSPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEM

• Full Name: cyclin G associated kinase
• Calculated Molecular Weight: 134 kDa, 143 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC008668
• Gene Symbol: GAK
• Gene ID (NCBI): 2580
• RRID: AB_2108368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14976
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAK(cyclin-G-associated kinase) belongs to the protein kinase superfamily. GAK plays a more positive role as a modulator of signal transduction, by relaying the phosphorylation signal from an unidentified tyrosine kinase to unidentified GAK phosphorylation targets(PMID:9013862). It may be acting as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by hsc70 in non-neuronal cells.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Biol Chem; Development of the non-receptor tyrosine kinase FER-targeting PROTACs as a potential strategy for antagonizing ovarian cancer cell motility and invasiveness; Authors - Yanchun Zhang
human	WB	Proc Natl Acad Sci U S A; Dynamic early recruitment of GAK-Hsc70 regulates coated pit maturation; Authors - Zhangping He ; KD Validated