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Galectin-3 Monoclonal antibody

Galectin-3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 60207-1-Ig

Synonyms

35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, Galectin-3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2



Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, A431 cells, MCF-7 cells, COLO 320 cells
Positive IHC detected inhuman thyroid cancer tissue, human colon tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells, human thyroid cancer tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60207-1-Ig is Galectin-3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Galectin-3 fusion protein Ag6753
Full Name lectin, galactoside-binding, soluble, 3
Calculated molecular weight 26 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC001120
Gene symbol LGALS3
Gene ID (NCBI) 3958
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a 31-kDa member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Protocols

Product Specific Protocols
WB protocol for Galectin-3 antibody 60207-1-IgDownload protocol
IHC protocol for Galectin-3 antibody 60207-1-IgDownload protocol
IF protocol for Galectin-3 antibody 60207-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma
mouseIF

Theranostics

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Authors - Gao-Shan Yang
humanWB,IHC

Cell Rep

Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.

Authors - Krishna S Tummala
humanWB,IF

Elife

Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy.

Authors - Vinay V Eapen
humanWB

Carbohydr Polym

Leukemia cells apoptosis by a newly discovered heterogeneous polysaccharide from Angelica sinensis (Oliv.) Diels.

Authors - Wenjuan Liu
mouseWB

Pharmacol Res

Yin-xing-tong-mai decoction attenuates atherosclerosis via activating PPARγ-LXRα-ABCA1/ABCG1 pathway.

Authors - Shasha Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Muhammad (Verified Customer) (09-13-2022)

Work best in HUVECs

  • Applications: Western Blot
  • Cell Tissue Type: HUVEC
FH

Noémie (Verified Customer) (10-20-2021)

I used this antibody for Western Blot against Gal3 in mamalian cells (U2OS), and did a validation by siRNA.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: U2OS cells
Galectin-3 Antibody Western Blot validation (1/1000 dilution) in U2OS cells (Cat no:60207-1-Ig)