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Galectin-3 Monoclonal antibody
Galectin-3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC
Conjugate
Unconjugated
CloneNo.
1C1B2
Cat no : 60207-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HSC-T6 cells, A431 cells, MCF-7 cells, COLO 320 cells |
| Positive IHC detected in | human thyroid cancer tissue, human colon tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, MCF-7 cells, human thyroid cancer tissue |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 4 publications below |
| IF | See 8 publications below |
Product Information
The immunogen of 60207-1-Ig is Galectin-3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Galectin-3 fusion protein Ag6753 |
| Full Name | lectin, galactoside-binding, soluble, 3 |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC001120 |
| Gene symbol | LGALS3 |
| Gene ID (NCBI) | 3958 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a 31-kDa member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Galectin-3 antibody 60207-1-Ig | Download protocol |
| IHC protocol for Galectin-3 antibody 60207-1-Ig | Download protocol |
| IF protocol for Galectin-3 antibody 60207-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta miR-155-dependent regulation of mammalian sterile 20-like kinase 2 (MST2) coordinates inflammation, oxidative stress and proliferation in vascular smooth muscle cells. | ||
Acta Biomater Tumor cell membrane-based peptide delivery system targeting the tumor microenvironment for cancer immunotherapy and diagnosis. | ||
Int J Mol Sci Galectin-3 Contributes to the Inhibitory Effect of lα,25-(OH)2D3 on Osteoclastogenesis.
| ||
Mol Med Rep Galectin‑3 facilitates the proliferation and migration of nasopharyngeal carcinoma cells via activation of the ERK1/2 and Akt signaling pathways, and is positively correlated with the inflammatory state of nasopharyngeal carcinoma. | ||
Am J Transl Res Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Noémie (Verified Customer) (10-20-2021) | I used this antibody for Western Blot against Gal3 in mamalian cells (U2OS), and did a validation by siRNA.
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