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Galectin-3 Monoclonal antibody
Galectin-3 Monoclonal Antibody for FC, IF, IHC, WB,ELISA
Cat no : 60207-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, HSC-T6 cells, A431 cells, MCF-7 cells, COLO 320 cells|
|Positive IHC detected in||human thyroid cancer tissue, human colon tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, human thyroid cancer tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60207-1-Ig is Galectin-3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Galectin-3 fusion protein Ag6753|
|Full Name||lectin, galactoside-binding, soluble, 3|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC001120|
|Gene ID (NCBI)||3958|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a 31-kDa member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).
|Product Specific Protocols|
|WB protocol for Galectin-3 antibody 60207-1-Ig||Download protocol|
|IHC protocol for Galectin-3 antibody 60207-1-Ig||Download protocol|
|IF protocol for Galectin-3 antibody 60207-1-Ig||Download protocol|
|FC protocol for Galectin-3 antibody 60207-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta
miR-155-dependent regulation of mammalian sterile 20-like kinase 2 (MST2) coordinates inflammation, oxidative stress and proliferation in vascular smooth muscle cells.
Tumor cell membrane-based peptide delivery system targeting the tumor microenvironment for cancer immunotherapy and diagnosis.
Mol Med Rep
Galectin‑3 facilitates the proliferation and migration of nasopharyngeal carcinoma cells via activation of the ERK1/2 and Akt signaling pathways, and is positively correlated with the inflammatory state of nasopharyngeal carcinoma.
Clin Oral Investig
Hypermethylation of IFN-γ in oral cancer tissues.
Am J Transl Res
Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages.
J Am Soc Hypertens
Galectin-3 mediates pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension.