GALNT12 Polyclonal antibody

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  WB analysis using 16415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16415-1-AP (GALNT12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GALNT12 Polyclonal Antibody for WB, ELISA

Cat No. 16415-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.4.1.41, GalNAc T12, GalNAc-T12, Polypeptide N-acetylgalactosaminyltransferase 12, pp GaNTase 12

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Tested Applications

• Positive WB detected in: HaCaT cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16415-1-AP targets GALNT12 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9571

  Product name: Recombinant human GALNT12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-272 aa of BC013945

  Sequence: MAGGLFAVSKKYFEYLGSYDTGMEVWGGENLEFSFRIWQCGGVLETHPCSHVGHVFPKQAPYSRNKALANSVRAAEVWMDEFKELYYHRNPRARLEPFGDVTERKQLRDKLQCKDFKWFLETVYPELHVPEDRPGFFGMLQNKGLTDYCFDYNPPDENQIVGHQVILYLCHGMGQNQFFEYTSQKEIRYNTHQPEGCIAVEAGMDTLIMHLCEETAPENQKFILQEDGSLFHEQSKKCVQAARKESSDSFVPLLRDCTNSDHQKWFFKERML

• Full Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 (GalNAc-T12)
• Calculated Molecular Weight: 581 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC013945
• Gene Symbol: GALNT12
• Gene ID (NCBI): 79695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IXK2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polypeptide N‑acetylgalactosaminyl transferase 12 (GALNT12) belongs to the uridine diphosphate N‑acetylgalactosamine gene family, which is involved in numerous biological processes of diseases, such as tumor progression. GALNT12 is highly expressed in the digestive tract and it is frequently downregulated in colorectal cancer (CRC) (PMID: 29095867). Genetic knockdown (KD) and knockout (KO) of GALNT12 in U87 MG, a representative GBM cell line with high GALNT12 expression, confirmed that GALNT12 deficiency leads to decreased cell proliferation, migration and invasion (PMID: 35461073).