GALNT4 Polyclonal antibody

• Featured Product
• KD/KO Validated

GALNT4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12897-1-AP

Synonyms

GALNAC T4, GalNAcT4, GALNT4, pp GaNTase 4

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Validation Data Gallery

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  WB analysis using 12897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12897-1-AP (GALNT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12897-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12897-1-AP (GALNT4 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

12897-1-AP targets GALNT4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GALNT4 fusion protein Ag3590
• Full Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4)
• Calculated Molecular Weight: 578 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC036390
• Gene Symbol: GALNT4
• Gene ID (NCBI): 8693
• RRID: AB_10643389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALNT4 is a member of N-acetylgalactosaminyltransferases (GALNTs) which are responsible for initiating step of mucin-type O-glycosylation. Desregulation of GALNTs expression can exert pivotal functions during malignant transformation. It has been reported that GALNT4 expression is markedly repressed in tumoral tissues and lower expression of GALNT4 is significant associated with the adverse survival, indicating its potential role as prognostic factor for cancers like hepatocellular carcinoma.

Publications

Species	Application	Title
human	WB,IHC	J Biol Chem; Loss of N-acetylgalactosaminyltransferase-4 orchestrate oncogenic microRNA-9 in hepatocellular carcinoma.; Authors - Yidong Liu ; KD Validated
mouse	WB,IHC	J Mol Cell Cardiol; GALNT4 primes monocytes adhesion and transmigration by regulating O-Glycosylation of PSGL-1 in atherosclerosis.; Authors - Zhishuai Ye ; KD Validated
mouse	WB	EMBO J; The disulfide catalyst QSOX1 maintains the colon mucosal barrier by regulating Golgi glycosyltransferases; Authors - Tal Ilani