Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, T-47D cells |
| Positive IHC detected in | human lung squamous cell cancer, human stomach cancer tissue, mouse salivary gland tissue, human placenta tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13671-1-AP targets GALNT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4652 Product name: Recombinant human GALNT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 323-622 aa of BC035822 Sequence: FDWSLTFGWETLPPHEKQRRKDETYPIKSPTFAGGLFSISKSYFEHIGTYDNQMEIWGGENVEMSFRVWQCGGQLEIIPCSVVGHVFRTKSPHTFPKGTSVIARNQVRLAEVWMDSYKKIFYRRNLQAAKMAQEKSFGDISERLQLREQLHCHNFSWYLHNVYPEMFVPDLTPTFYGAIKNLGTNQCLDVGENNRGGKPLIMYSCHGLGGNQYFEYTTQRDLRHNIAKQLCLHVSKGALGLGSCHFTGKNSQVPKDEEWELAQDQLIRNSGSGTCLTSQDKKPAMAPCNPSDPHQLWLFV Predict reactive species |
| Full Name | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6) |
| Calculated Molecular Weight | 622 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC035822 |
| Gene Symbol | GALNT6 |
| Gene ID (NCBI) | 11226 |
| RRID | AB_3669168 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NCL4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GALNT6 (polypeptide N-acetylgalactosaminyltransferase 6, GalNAc-T6), which is involved in the initiation of O-glycosylation, has been reported to play crucial roles in mammary carcinogenesis through binding to several substrates (PMID: 32393740). GALNT6 is upregulated in breast, ovarian, renal cell, lung and pancreatic cancers (PMID: 20215525). Overexpression of GRP78 could enhance Golgi-to-ER relocation of GALNT6 (PMID: 28110670).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GALNT6 antibody 13671-1-AP | Download protocol |
| IHC protocol for GALNT6 antibody 13671-1-AP | Download protocol |
| WB protocol for GALNT6 antibody 13671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GALNT6 Polyclonal antibody (catalog number 13671-1-AP) has 1 total citation. It appears in the journal Life Sciences (PMID 42628639), in a study titled "GALNT6-mediated O-glycosylation suppresses NLRP3 inflammasome activation to alleviate atopic dermatitis." The associated research field is immunology/inflammation, specifically O-glycosylation's role in NLRP3 inflammasome regulation and atopic dermatitis.
| Species | Application | Title |
|---|---|---|
Life Sci GALNT6-mediated O-glycosylation suppresses NLRP3 inflammasome activation to alleviate atopic dermatitis. |
Reviews
What customers say
The product received a 5-star rating from a user who tested it on human cell lines and tissue. The reviewer reported that it works well for both Western Blot and Immunohistochemistry applications, indicating reliable performance across these two common techniques. Overall, the feedback is positive, with no issues or limitations mentioned.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jennifer (Verified Customer) (04-22-2026) | Works well for western blotting and IHC.
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