Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, human brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13962-1-AP targets GALNT7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4917 Product name: Recombinant human GALNT7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 308-657 aa of BC047468 Sequence: VNWYAPLVAPISKDRTICTVPLIDVINGNTYEIIPQGGGDEDGYARGAWDWSMLWKRVPLTPQEKRLRKTKTEPYRSPAMAGGLFAIEREFFFELGLYDPGLQIWGGENFEISYKIWQCGGKLLFVPCSRVGHIYRLEGWQGNPPPIYVGSSPTLKNYVRVVEVWWDEYKDYFYASRPESQALPYGDISELKKFREDHNCKSFKWFMEEIAYDITSHYPLPPKNVDWGEIRGFETAYCIDSMGKTNGGFVELGPCHRMGGNQLFRINEANQLMQYDQCLTKGADGSKVMITHCNLNEFKEWQYFKNLHRFTHIPSGKCLDRSEVLHQVFISNCDSSKTTQKWEMNNIHSV Predict reactive species |
| Full Name | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) |
| Calculated Molecular Weight | 75.4 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC047468 |
| Gene Symbol | GALNT7 |
| Gene ID (NCBI) | 51809 |
| RRID | AB_2109009 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86SF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GALNT7 is a member of GALNT family that are glycotransferases involved in oligosaccharide biosynthesis. Aberrant glycosylation has been linked to tumor development and progression. Increased expression of GALNT7 has been observed in cervical cancer cells. (PMID: 22399294)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GALNT7 antibody 13962-1-AP | Download protocol |
| WB protocol for GALNT7 antibody 13962-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MUC2 antibody (Cat# 13962-1-AP) has 3 citations. These appear in J Pathol Clin Res, Eur J Histochem, and Am J Transl Res. The associated research fields include salivary duct carcinoma (cancer glycocalyx/MUC1), histological characterization of the musk gland in forest musk deer, and inflammatory bowel disease (IBD) involving MUC2 synthesis via the ANRIL-miR-34a axis. Applications cited are IHC and WB.
| Species | Application | Title |
|---|---|---|
J Pathol Clin Res Targeting the cancer glycocalyx in salivary duct carcinoma: tumor-associated mucin 1 (Tn-MUC1) as a novel cell surface marker | ||
Eur J Histochem Histological and histochemical characterization of the musk gland in forest musk deer (Moschus berezovskii): a preliminary study | ||
Am J Transl Res Resveratrol enhances MUC2 synthesis via the ANRIL-miR-34a axis to mitigate IBD |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used it on TRAMP-C2 cells across multiple applications, including immunohistochemistry, immunofluorescence, flow cytometry, cell culture, and protein purification, at a 1:200 dilution. The only comment noted a 3-hour incubation. Overall, the feedback is positive, indicating the antibody performed well across a broad range of techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Maggie (Verified Customer) (11-03-2023) | 3 hours incubation
 |
