GALNT9 Polyclonal antibody

GALNT9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25967-1-AP

Synonyms

GALNAC T9, GALNT9, pp GaNTase 9

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Validation Data Gallery

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  WB analysis of mouse brain using 25967-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25967-1-AP (GALNT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 25967-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25967-1-AP (GALNT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 25967-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25967-1-AP (GALNT9 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of rat brain using 25967-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25967-1-AP (GALNT9 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, C6 cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 25967-1-AP is GALNT9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GALNT9 fusion protein Ag23233
• Full Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 9 (GalNAc-T9)
• Observed Molecular Weight: 66-68 kDa
• GenBank Accession Number: BC157845
• Gene Symbol: GALNT9
• Gene ID (NCBI): 50614
• RRID: AB_2880313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Cell Mol Med; Genome-wide methylomic analyses identify prognostic epigenetic signature in lower grade glioma.; Authors - Wenna Guo