GALNTL2 Polyclonal antibody

GALNTL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16900-1-AP

Synonyms

GalNAc T like protein 2, GALNT15, GALNT7, GALNTL2, PIH5, pp GaNTase like protein 2

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Validation Data Gallery

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  WB analysis of HEK-293 using 16900-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16900-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse small intestine using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human liver tissue
• Positive IHC detected in: mouse small intestine tissue, human kidney tissue, human testis tissue, human placenta tissue, human brain tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16900-1-AP targets GALNTL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GALNTL2 fusion protein Ag10434
• Full Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2
• Calculated Molecular Weight: 639 aa, 73 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC014789
• Gene Symbol: GALNTL2
• Gene ID (NCBI): 117248
• RRID: AB_2263208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.