GAMT Polyclonal antibody

GAMT Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10880-1-AP

Synonyms

GAMT, PIG2, TP53I2



Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue, rat liver tissue, HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman liver tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10880-1-AP targets GAMT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAMT fusion protein Ag1327
Full Name guanidinoacetate N-methyltransferase
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC016760
Gene Symbol GAMT
Gene ID (NCBI) 2593
RRIDAB_2109304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.

Protocols

Product Specific Protocols
WB protocol for GAMT antibody 10880-1-APDownload protocol
IHC protocol for GAMT antibody 10880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Agric Food Chem

Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.

Authors - Sho Nishikawa
mouseWB

Toxicol Appl Pharmacol

Cytochalasin Q exerts anti-melanoma effect by inhibiting creatine kinase B.

Authors - Yi Lu
ratWB

Ann Transl Med

Transcriptomics combined with metabolomics analysis of the mechanism of agmatine in the treatment of septic liver injury.

Authors - Ling Huang
mouseWB,IF

Brain Res

GATM and GAMT synthesize creatine locally throughout the mammalian body and within oligodendrocytes of the brain.

Authors - Steven Andrew Baker
zebrafish

J Fish Biol

Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio.

Authors - Wang L L
mouseWB

Redox Biol

Unbalanced redox status network as an early pathological event in congenital cataracts

Authors - Eloy Bejarano

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Werner (Verified Customer) (11-28-2017)

13% SDS-PAGE, 10 ug total liver lysate loaded, overnight incubation with primary antibody at 4°C

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse liver
GAMT Antibody Western Blot, validation (1:2000 dilution) in Mouse liver (Cat no:10880-1-AP)