Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse liver tissue, rat liver tissue, HepG2 cells, SH-SY5Y cells|
|Positive IHC detected in||human liver tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10880-1-AP targets GAMT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GAMT fusion protein Ag1327|
|Full Name||guanidinoacetate N-methyltransferase|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC016760|
|Gene ID (NCBI)||2593|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.
J Agric Food Chem
Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.
Toxicol Appl Pharmacol
Cytochalasin Q exerts anti-melanoma effect by inhibiting creatine kinase B.
Ann Transl Med
Transcriptomics combined with metabolomics analysis of the mechanism of agmatine in the treatment of septic liver injury.
GATM and GAMT synthesize creatine locally throughout the mammalian body and within oligodendrocytes of the brain.
J Fish Biol
Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Werner (Verified Customer) (11-28-2017)
13% SDS-PAGE, 10 ug total liver lysate loaded, overnight incubation with primary antibody at 4°C