|Positive WB detected in||mouse liver tissue, HEK-293 cells, human brain tissue, human heart tissue, mouse brain tissue, rat liver tissue|
|Positive IHC detected in||human liver tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10880-1-AP targets GAMT in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
|Tested Reactivity||human, mouse, rat, zebrafish|
|Cited Reactivity||mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GAMT fusion protein Ag1327|
|Full Name||guanidinoacetate N-methyltransferase|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC016760|
|Gene ID (NCBI)||2593|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.
J Fish Biol
Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio.
J Agric Food Chem
Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Werner (Verified Customer) (11-28-2017)
13% SDS-PAGE, 10 ug total liver lysate loaded, overnight incubation with primary antibody at 4°C