Tested Applications
| Positive WB detected in | HEK-293 cells, mouse liver tissue, rat liver tissue, HepG2 cells, SH-SY5Y cells |
| Positive IHC detected in | human liver tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
10880-1-AP targets GAMT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GAMT fusion protein Ag1327 Predict reactive species |
| Full Name | guanidinoacetate N-methyltransferase |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC016760 |
| Gene Symbol | GAMT |
| Gene ID (NCBI) | 2593 |
| RRID | AB_2109304 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14353 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GAMT antibody 10880-1-AP | Download protocol |
| IHC protocol for GAMT antibody 10880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway. | ||
Toxicol Appl Pharmacol Cytochalasin Q exerts anti-melanoma effect by inhibiting creatine kinase B. | ||
Ann Transl Med Transcriptomics combined with metabolomics analysis of the mechanism of agmatine in the treatment of septic liver injury. | ||
Brain Res GATM and GAMT synthesize creatine locally throughout the mammalian body and within oligodendrocytes of the brain. | ||
J Fish Biol Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio. | ||
Redox Biol Unbalanced redox status network as an early pathological event in congenital cataracts |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Werner (Verified Customer) (11-28-2017) | 13% SDS-PAGE, 10 ug total liver lysate loaded, overnight incubation with primary antibody at 4°C
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