GAMT Polyclonal antibody

GAMT Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10880-1-AP

Synonyms

GAMT, PIG2, TP53I2



Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, human brain tissue, human heart tissue, mouse brain tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10880-1-AP targets GAMT in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivity mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAMT fusion protein Ag1327
Full Name guanidinoacetate N-methyltransferase
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC016760
Gene symbol GAMT
Gene ID (NCBI) 2593
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.

Protocols

Product Specific Protocols
WB protocol for GAMT antibody 10880-1-APDownload protocol
IHC protocol for GAMT antibody 10880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafish

J Fish Biol

Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio.

Authors - Wang L L
mouseWB

J Agric Food Chem

Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.

Authors - Sho Nishikawa

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Werner (Verified Customer) (11-28-2017)

13% SDS-PAGE, 10 ug total liver lysate loaded, overnight incubation with primary antibody at 4°C

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse liver
GAMT Antibody Western Blot, validation (1:2000 dilution) in Mouse liver (Cat no:10880-1-AP)