GAMT Polyclonal antibody

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  WB analysis using 10880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10880-1-AP (GAMT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 10880-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10880-1-AP (GAMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10880-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10880-1-AP (GAMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 40x lens).

GAMT Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10880-1-AP

Publications(8)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

GAMT, PIG2, TP53I2

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue, rat liver tissue, HepG2 cells, SH-SY5Y cells
• Positive IHC detected in: human liver tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

10880-1-AP targets GAMT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAMT fusion protein Ag1327
• Full Name: guanidinoacetate N-methyltransferase
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC016760
• Gene Symbol: GAMT
• Gene ID (NCBI): 2593
• RRID: AB_2109304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14353
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.

Publications

Species	Application	Title
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa
mouse	WB	Toxicol Appl Pharmacol; Cytochalasin Q exerts anti-melanoma effect by inhibiting creatine kinase B.; Authors - Yi Lu
rat	WB	Ann Transl Med; Transcriptomics combined with metabolomics analysis of the mechanism of agmatine in the treatment of septic liver injury.; Authors - Ling Huang
mouse	WB,IF	Brain Res; GATM and GAMT synthesize creatine locally throughout the mammalian body and within oligodendrocytes of the brain.; Authors - Steven Andrew Baker
zebrafish		J Fish Biol; Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio.; Authors - Wang L L
mouse	WB	Redox Biol; Unbalanced redox status network as an early pathological event in congenital cataracts; Authors - Eloy Bejarano

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Werner (Verified Customer) (11-28-2017)

13% SDS-PAGE, 10 ug total liver lysate loaded, overnight incubation with primary antibody at 4°C

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver

GAMT Antibody Western Blot, validation (1:2000 dilution) in Mouse liver (Cat no:10880-1-AP)