GAN Polyclonal antibody

GAN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14305-1-AP

Synonyms

GAN, GAN1, gigaxonin, Kelch like protein 16, KLHL16

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Validation Data Gallery

• 

  WB analysis of mouse brain using 14305-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14305-1-AP (GAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14305-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14305-1-AP (GAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14305-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14305-1-AP (GAN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14305-1-AP targets GAN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAN fusion protein Ag5421
• Full Name: gigaxonin
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC044840
• Gene Symbol: GAN
• Gene ID (NCBI): 8139
• RRID: AB_2109308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Development; MicroRNA deep sequencing in two adult stem cell populations identifies miR-501 as a novel regulator of myosin heavy chain during muscle regeneration.; Authors - Amir Mizbani
mouse	WB	J Invest Dermatol; Excess KLHL24 impairs skin wound healing by degradation of vimentin; Authors - Yihe Liu