GAN Polyclonal antibody

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• GAN Polyclonal antibody (14305-1-AP)

GAN Polyclonal Antibody for WB, ELISA

Cat No. 14305-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GAN, GAN1, gigaxonin, Kelch like protein 16, KLHL16

• 

  WB analysis of mouse brain using 14305-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14305-1-AP (GAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 14305-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14305-1-AP (GAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 14305-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14305-1-AP (GAN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14305-1-AP targets GAN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5421

  Product name: Recombinant human GAN protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 248-597 aa of BC044840

  Sequence: CSNIPLSQPQQGEAMLANFKPRGYSECIVTVGGEERVSRKPTAAMRCMCPLYDPNRQLWIELAPLSMPRINHGVLSAEGFLFVFGGQDENKQTLSSGEKYDPDANTWTALPPMNEARHNFGIVEIDGMLYILGGEDGEKELISMECYDIYSKTWTKQPDLTMVRKIGCYAAMKKKIYAMGGGSYGKLFESVECYDPRTQQWTAICPLKERRFGAVACGVAMELYVFGGVRSREDAQGSEMVTCKSEFYHDEFKRWIYLNDQNLCIPASSSFVYGAVPIGASIYVIGDLDTGTNYDYVREFKRSTGTWHHTKPLLPSDLRRTGCAALRIANCKLFRLQLQQGLFRIRVHSP

• Full Name: gigaxonin
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC044840
• Gene Symbol: GAN
• Gene ID (NCBI): 8139
• RRID: AB_2109308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2C0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The GAN polyclonal antibody (Cat# 14305-1-AP) has 2 citations. One appeared in Journal of Investigative Dermatology (2023), studying how excess KLHL24 impairs skin wound healing via vimentin degradation in mouse models. The other was published in Development (2016), identifying miR-501 as a regulator of myosin heavy chain during human muscle regeneration. Both citations used the antibody for Western blot applications, spanning research fields of dermatology/wound healing and muscle stem cell biology.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Invest Dermatol; Excess KLHL24 impairs skin wound healing by degradation of vimentin; Authors - Yihe Liu
human	WB	Development; MicroRNA deep sequencing in two adult stem cell populations identifies miR-501 as a novel regulator of myosin heavy chain during muscle regeneration.; Authors - Amir Mizbani