GAP43 Polyclonal antibody

GAP43 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16971-1-AP

Synonyms

Axonal membrane protein GAP 43, B 50, GAP43, growth associated protein 43, Neural phosphoprotein B 50, Neuromodulin, PP46



Tested Applications

Positive WB detected inmouse brain tissue, rat lung tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16971-1-AP targets GAP43 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity canine, hamster, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAP43 fusion protein Ag9294
Full Name growth associated protein 43
Calculated molecular weight 238 aa, 25 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC007936
Gene symbol GAP43
Gene ID (NCBI) 2596
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The neuronal growth-associated protein GAP43 is also known as neuromodulin, B-50, P-57, F1 and pp46. Deficiency of GAP43 in mice results in death early in the postnatal period. GAP43 is one of the main substrates for protein kinase C in the brain. GAP43 is an intracellular growth-associated protein that appears to assist neuronal pathfinding and branching during development and regeneration, and may contribute to presynaptic membrane changes in the adult, leading to the neurotransmitter release, endocytosis and synaptic vesicle recycling, long-term potentiation, spatial memory formation, and learning. The predicated molecular weight of about 25 kDa is much lower than the apparent observed molecular weight of 43 kDa on SDS-PAGE gels, and this occurs because the highly charged nature of GAP43 causes it to bind less than the average amount of SDS per amino acid, and because the protein has an elongated structure.

Protocols

Product Specific Protocols
WB protocol for GAP43 antibody 16971-1-APDownload protocol
IHC protocol for GAP43 antibody 16971-1-APDownload protocol
IP protocol for GAP43 antibody 16971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
hamsterWB

Int J Mol Sci

PMID: 30845718

Authors - Syed Zahid Ali Shah
ratWB,IF

Ann Transl Med

Catalpol induces cell activity to promote axonal regeneration via the PI3K/AKT/mTOR pathway in vivo and in vitro stroke model.

Authors - Jinghuan Wang
ratIHC

Stem Cells Int

Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo.

Authors - Rui D Alvites
ratIHC

Neural Regen Res

Bone marrow mesenchymal stem cells repair spinal cord ischemia/reperfusion injury by promoting axonal growth and anti-autophagy.

Authors - Fei Yin
ratWB

Acta Histochem

Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.

Authors - Yanbin Wang
ratWB,IF

Neurochem Res

Runx2 was Correlated with Neurite Outgrowth and Schwann Cell Differentiation, Migration After Sciatic Nerve Crush.

Authors - Dazhi Ding

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/500 GAP43. Very strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: Human primary cortical neurons
GAP43 Antibody Immunohistochemistry validation (1/500 dilution) in Human primary cortical neurons (Cat no:16971-1-AP)