Tested Applications
| Positive WB detected in | mouse brain tissue, HEK-293 cells, rat brain tissue, NIH/3T3 cells, HSC-T6 cells, K-562 cells, Jurakat cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81640-5-RR targets GAPDH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0766 Product name: Recombinant human GAPDH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC004109 Sequence: MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE Predict reactive species |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC004109 |
| Gene Symbol | GAPDH |
| Gene ID (NCBI) | 2597 |
| RRID | AB_3086559 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04406 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GAPDH antibody 81640-5-RR | Download protocol |
| WB protocol for GAPDH antibody 81640-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GAPDH Recombinant Monoclonal Antibody (Cat# 81640-5-RR) has 141 citations. These appear across high-impact journals including Nature Biomedical Engineering, Nature Communications, Autophagy, Journal of Advanced Research, Journal of Nanobiotechnology, Advanced Science, Cell Reports Medicine, Journal of Experimental Medicine, Phytomedicine, Free Radical Biology and Medicine, EMBO Reports, FASEB Journal, and Scientific Reports, among many others. Research fields span oncology, neurodegeneration, immunology and inflammation, ferroptosis and cell death, fibrosis, diabetes, and pharmacology, with Western blot as the dominant application.
| Species | Application | Title |
|---|---|---|
Nat Biomed Eng Targeted clearance of extracellular Tau using aptamer-armed monocytes alleviates neuroinflammation in mice with Alzheimer's disease. | ||
Autophagy AARS2-mediated lactylation of ULK1 promotes autophagy-dependent progression of clear cell renal cell carcinoma. | ||
Nat Commun IRF2BP2 binds to a conserved RxSVI motif of protein partners and regulates megakaryocytic differentiation | ||
J Adv Res Diosgenin activates ERα-dependent PI3K/AKT/GSK3β signaling to enhance brain-derived serotonin and alleviate postmenopausal bone loss. | ||
J Adv Res Fibroblast-derived BMP5 promotes tendon adhesion by inducing bone marrow mesenchymal stromal cells to differentiate into myofibroblasts. | ||
J Nanobiotechnology PDLIM4 drives gastric cancer malignant progression and cisplatin resistance by inhibiting HSP70 ubiquitination and degradation via competitive interaction with STUB1. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot at a 1:5000 dilution, tested on H9C2 cells and liver tissue. One reviewer noted that the antibody produced clear, well-defined bands at the expected molecular weight. Overall, the feedback highlights reliable specificity and strong signal quality in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
YINGJIAN (Verified Customer) (07-24-2025) |
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YINGJIAN (Verified Customer) (07-24-2025) | This antibody provided clear and well-defined bands at the expected molecular weight in Western blot assays
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