GAPDHS Recombinant monoclonal antibody

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  WB analysis using 83290-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83290-3-RR (GAPDHS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83290-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83290-3-RR against Human GAPDHS were performed. The affinity constant is below 1 pM.

GAPDHS Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83290-3-RR

Clone No.240277F1

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

GAPD2, GAPDH 2, GAPDH2, GAPDHS, GAPDS, HSD 35

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, NIH/3T3 cells, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83290-3-RR targets GAPDHS in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4988

  Product name: Recombinant human GAPDHS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 58-408 aa of BC036373

  Sequence: LPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK

• Full Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC036373
• Gene Symbol: GAPDHS
• Gene ID (NCBI): 26330
• RRID: AB_3670957
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O14556
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, testis-specific) is also named as GAPD2, GAPDH2, GAPDS and belongs to the glyceraldehyde-3-phosphate dehydrogenase family. It may play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. GAPDHS is a promising contraceptive target because it is specific to male germ cells, essential for sperm motility and male fertility, and well suited to pharmacological inhibition(PMID:20828617).