Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11711-1-AP targets GAR1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2282 Product name: Recombinant human GAR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC003413 Sequence: MSFRGGGRGGFNRGGGGGGFNRGGSSNHFRGGGGGGGGGNFRGGGRGGFGRGGGRGGFNKGQDQGPPERVVLLGEFLHPCEDDIVCKCTTDENKVPYFNAPVYLENKEQIGKVDEIFGQLRDFYFSVKLSENMKASSFKKLQKFYIDPYKLLPLQRFLPRPPGEKGPPRGGGRGGRGGGRGGGGRGGGRGGGFRGGRGGGGGGFRGGRGGGFRGRGH Predict reactive species |
| Full Name | GAR1 ribonucleoprotein homolog (yeast) |
| Calculated Molecular Weight | 217 aa, 22 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC003413 |
| Gene Symbol | GAR1 |
| Gene ID (NCBI) | 54433 |
| RRID | AB_2081845 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NY12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GAR1 antibody 11711-1-AP | Download protocol |
| IP protocol for GAR1 antibody 11711-1-AP | Download protocol |
| WB protocol for GAR1 antibody 11711-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GAR1 Polyclonal antibody (Cat# 11711-1-AP) has a total of 17 citations. These appear in journals including Cell, Molecular Cell, Nucleic Acids Research, Genome Biology, Cell Reports, Human Molecular Genetics, Cell Death & Disease, Frontiers in Cell and Developmental Biology, Pathology, and others. The associated research fields encompass H/ACA ribonucleoprotein biogenesis, telomere maintenance, snoRNA function, DNA damage repair, cell proliferation, and clinical pathology.
| Species | Application | Title |
|---|---|---|
Cell snoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification | ||
Mol Cell The human base excision repair enzyme SMUG1 directly interacts with DKC1 and contributes to RNA quality control. | ||
Cell Death Dis Biallelic mutations in WRAP53 result in dysfunctional telomeres, Cajal bodies and DNA repair, thereby causing Hoyeraal-Hreidarsson syndrome. | ||
Genome Biol Mapping snoRNA-target RNA interactions in an RNA-binding protein-dependent manner with chimeric eCLIP | ||
Genome Biol Human dyskerin binds to cytoplasmic H/ACA-box-containing transcripts affecting nuclear hormone receptor dependence |
