GARNL1 Polyclonal antibody

GARNL1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29847-1-AP

Synonyms

DKFZp566D133, DKFZp667F074, GARNL1, GRIPE, KIAA0884, p240, RALGAPA1, Tuberin like protein 1, TULIP1

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Validation Data Gallery

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  WB analysis using 29847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29847-1-AP (GARNL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 29847-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GARNL1 antibody (29847-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29847-1-AP targets GARNL1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GARNL1 fusion protein Ag31518
• Full Name: GTPase activating Rap/RanGAP domain-like 1
• Calculated Molecular Weight: 230KD
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: NM_014990.3
• Gene Symbol: GARNL1
• Gene ID (NCBI): 253959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, as a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).The 100 kDa band maybe an isoform or fragment (BAA74907, 943aa).