GART Polyclonal antibody

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GART Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13659-1-AP Copied!

Publications (21)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

5'-phosphoribosylglycinamide transformylase, AIR synthase, AIRS, GAR transformylase, GARS

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  WB analysis using 13659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 13659-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 13659-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 13659-1-AP

  IP result of anti-GART (IP:13659-1-AP, 4ug; Detection:13659-1-AP 1:500) with HepG2 cells lysate 1600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 13659-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13659-1-AP (GART antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 13659-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13659-1-AP (GART antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 13659-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13659-1-AP (GART antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells, L02 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13659-1-AP targets GART in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4555

  Product name: Recombinant human GART protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-318 aa of BC038958

  Sequence: MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLTKNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLD

• Full Name: phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
• Calculated Molecular Weight: 1010 aa, 108 kDa
• Observed Molecular Weight: 108 kDa, 46 kDa
• GenBank Accession Number: BC038958
• Gene Symbol: GART
• Gene ID (NCBI): 2618
• RRID: AB_2107937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22102
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.

Publications

What published studies show

The GART Polyclonal antibody (Cat# 13659-1-AP) has 21 citations. These appear in high-impact journals including Nature, Science, Cell, Nature Metabolism, Nature Communications, Molecular Cell, Advanced Science, Oncogene, EMBO Journal, Cell Reports, European Journal of Pharmacology, Chemical Biology & Interactions, Molecular Nutrition & Food Research, and bioRxiv. Research fields span purine/nucleotide metabolism, mTORC1 signaling, cancer (breast, colorectal, renal, myeloma, gastric, liver), tumor stemness, hyperuricemia, and immune/metabolic regulation.

Automatically generated from the 21 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Nutrient requirements of organ-specific metastasis in breast cancer.; Authors - Keene L. Abbott ; KO Validated
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
mouse,human	WB	Cell; De novo and salvage purine synthesis pathways across tissues and tumors; Authors - Diem H Tran
human	WB	Nat Metab; Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.; Authors - Diem H Tran
mouse	WB	Nat Commun; Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.; Authors - Mona Hoseini Soflaee
human	WB,IF	Nat Commun; Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues.; Authors - S. Kourtis

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

The single available review rates the product 4 out of 5 stars. The reviewer used it for Western Blot at a 1:500 dilution, testing on HepG2 cells, mouse liver, mouse lung, and mouse hippocampus tissues. The overall feedback was positive, with the reviewer simply noting that it "works well."

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HepG2, mouse liver, mouse lung, mouse hippocampus

GART Antibody Western Blot, validation (1:500 dilution) in HepG2, mouse liver, mouse lung, mouse hippocampus (Cat no:13659-1-AP)