GAS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GAS1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17903-1-AP

Synonyms

GAS 1, GAS1, growth arrest 1

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 17903-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17903-1-AP (GAS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 17903-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17903-1-AP (GAS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17903-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17903-1-AP (GAS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

The immunogen of 17903-1-AP is GAS1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAS1 fusion protein Ag12275
• Full Name: growth arrest-specific 1
• Calculated Molecular Weight: 345 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC074908
• Gene Symbol: GAS1
• Gene ID (NCBI): 2619
• RRID: AB_2878463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAS1 is involved in growth suppression. It is an integral plasma membrane protein whose expression is linked to growth arrest. It can be used as a novel therapeutic candidate for gastric cancer.

Publications

Species	Application	Title
human,mouse	WB,IF	Cancer Commun (Lond); Bifidobacterium adolescentis orchestrates CD143+ cancer-associated fibroblasts to suppress colorectal tumorigenesis by Wnt signaling-regulated GAS1; Authors - Shujie Chen
human	IHC	Sci Rep; Gene-Related Response of Basal Cell Carcinoma to Biologic Treatment with Vismodegib.; Authors - Amir Sternfeld
human	WB,IF	Exp Ther Med; microRNA-mediated GAS1 downregulation promotes the proliferation of synovial fibroblasts by PI3K-Akt signaling in osteoarthritis.; Authors - Chuan Dong ; KD Validated
human	WB	FASEB Bioadv; Critical cysteines in the functional interaction of adenylyl cyclase isoform 6 with Gαs.; Authors - Anjali Y Bhagirath
human,mouse	WB,IHC,IF	Int J Med Sci; GAS1 Promotes Ferroptosis of Liver Cells in Acetaminophen-Induced Acute Liver Failure; Authors - Jinqiu Tao ; KD Validated