GATA3 Monoclonal antibody

GATA3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Conjugate

Unconjugated

Publications

16

CloneNo.

1B11A8

Reviews (1)

Cat no : 66400-1-Ig

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

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Validation Data Gallery

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  WB analysis of MCF-7 using 66400-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66400-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66400-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66400-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (66400-1-Ig, Clone: 1B11A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66400-1-Ig(GATA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, Jurkat cells, RAW 264.7 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: MCF-7 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

66400-1-Ig targets GATA3 in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GATA3 fusion protein Ag0174
• Full Name: GATA binding protein 3
• Calculated Molecular Weight: 443 aa, 48 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC003070
• Gene Symbol: GATA3
• Gene ID (NCBI): 2625
• RRID: AB_2881774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.

Publications

Species	Application	Title
human	WB,IHC	Drug Resist Updat; GATA3 mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction in breast cancer cells; Authors - Zhen Zhu
human	WB	Cardiovasc Diabetol; BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling; Authors - Xinyue Huang
pig	WB	J Ethnopharmacol; Therapeutic effects and mechanisms study of Hanchuan Zupa Granule in a Guinea pig model of cough variant asthma.; Authors - Pengfei Li
mouse	WB	Pharm Biol; Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway; Authors - Muhua Huang
mouse	WB	Int Arch Allergy Immunol; Lack of WDFY4 Aggravates Ovalbumin-Induced Asthma via Enhanced Th2 Cell Differentiation.; Authors - Yan Li
mouse	WB,IF	Evid Based Complement Alternat Med; Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway.; Authors - Zhaoyu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Renan (Verified Customer) (08-18-2022)

The fluorescence staining was noticeable, but the intensity was not so high, after using a tyramide signal amplification (TSA) protocol. The picture shows a lung lymphoid follicle, part of a 4-um FFPE tissue section. Heat-induced antigen retrieval was done with TRIS-EDTA buffer (pH 9.0) and GATA-3 was stained using a tyramide-conjugated Alexa Fluor 488 dye. CD3 is stained in yellow.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Lung lymphoid follicle

GATA3 Antibody Immunohistochemistry,Immunofluorescence validation (1:100 dilution) in Lung lymphoid follicle (Cat no:66400-1-Ig)