CoraLite®594-conjugated GATA3 Monoclonal antibody

GATA3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B11A8

Cat no : CL594-66400

Synonyms

GATA binding factor 3, GATA binding protein 3, GATA3, HDR

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Validation Data Gallery

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  IF Staining of MCF-7 using CL594-66400

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 GATA3 antibody (CL594-66400, Clone: 1B11A8 ) at dilution of 1:800, CL488-Phalloidin (green).

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  IF Staining of human breast cancer using CL594-66400

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66400 (GATA3 antibody) at dilution of 1:50.

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  IF Staining of human breast cancer using CL594-66400

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66400 (GATA3 antibody) at dilution of 1:50.

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Tested Applications

• Positive IF detected in: MCF-7 cells, human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66400 targets GATA3 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GATA3 fusion protein Ag0174
• Full Name: GATA binding protein 3
• Calculated Molecular Weight: 443 aa, 48 kDa
• GenBank Accession Number: BC003070
• Gene Symbol: GATA3
• Gene ID (NCBI): 2625
• RRID: AB_2883576
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.