GATA5 Polyclonal antibody

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  WB analysis of HepG2 using 55433-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55433-1-AP (GATA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55433-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55433-1-AP (GATA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 55433-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55433-1-AP (GATA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 55433-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55433-1-AP (GATA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 55433-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55433-1-AP (GATA5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 55433-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55433-1-AP (GATA5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 55433-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA5 antibody (55433-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

GATA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55433-1-AP

Publications(4)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Transcription factor GATA-5, Transcription factor GATA 5, GATA-binding factor 5, GATA binding factor 5

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

55433-1-AP targets GATA5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GATA binding protein 5
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: NM_080473
• Gene Symbol: GATA5
• Gene ID (NCBI): 140628
• RRID: AB_2881330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BWX5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IP	Cancers (Basel); Overexpression of GATA5 Inhibits Prostate Cancer Progression by Regulating PLAGL2 via the FAK/PI3K/AKT Pathway.; Authors - Qinghua Wang
human,mouse	WB,IF,IP	Biochem Biophys Res Commun; Endothelial GATA5 positively regulates angiogenesis via cathepsin S-mediated Angpt2/Flk1 and MMP2/9 signaling pathways.; Authors - Anmin Ren
human	WB	Nat Commun; Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy; Authors - Wenjing Bai
mouse	WB	Int J Biol Sci; Cardiovascular Mettl3 Deficiency Causes Congenital Cardiac Defects and Postnatal Lethality in Mice; Authors - Qianqian Feng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (01-26-2024)

Works well when diluted in 1X TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue