GBA Polyclonal antibody

GBA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27972-1-AP

Synonyms

Acid beta glucosidase, Alglucerase, Beta glucocerebrosidase, GBA, GBA1, GC, GCB, GLUC, Glucosylceramidase, Imiglucerase

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Validation Data Gallery

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  WB analysis using 27972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27972-1-AP (GBA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 27972-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27972-1-AP (GBA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 27972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells
• Positive IHC detected in: mouse brain tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

27972-1-AP targets GBA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBA fusion protein Ag27388
• Full Name: glucosidase, beta; acid (includes glucosylceramidase)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC003356
• Gene Symbol: GBA
• Gene ID (NCBI): 2629
• RRID: AB_2881024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins; Authors - Fatema Akter
mouse	WB	Sci Total Environ; Manganese disrupts the maturation and degradation of axonal autophagosome leading to hippocampal synaptic toxicity in mice via the activation of LRRK2 on phosphorylation of Rab10; Authors - Zhuo Ma