Tested Applications
| Positive WB detected in | human placenta tissue, HeLa cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17164-1-AP targets GBA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10606 Product name: Recombinant human GBA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 574-927 aa of BC011363 Sequence: RRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQNFLKDMWPVCLAVMESEMKFDKDHDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQDKFSSILSRGQEAYERLLWNGRYYNYDSSSRPQSRSVMSDQCAGQWFLKACGLGEGDTEVFPTQHVVRALQTIFELNVQAFAGGAMGAVNGMQPHGVPDKSSVQSDEVWVGVVYGLAATMIQEGLTWEGFQTAEGCYRTVWERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLALQQQQHKKASWPKVKQGTGLRTGPMFGPKEAMANLSPE Predict reactive species |
| Full Name | glucosidase, beta (bile acid) 2 |
| Calculated Molecular Weight | 927 aa, 105 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC011363 |
| Gene Symbol | GBA2 |
| Gene ID (NCBI) | 57704 |
| RRID | AB_2109077 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCG7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GBA2(Non-lysosomal glucosylceramidase) is also named as KIAA1605,NLGase and belongs to the non-lysosomal glucosylceramidase family.It catalyzes the conversion of glucosylceramide to free glucose and ceramide and is involved in sphingomyelin generation and prevention of glycolipid accumulation.GBA2 contains a putative membrane-spanning domain and 2 potential N-glycosylation sites. However, glycosidase treatment indicated that GBA2 is not a glycoprotein(PMID:11489889).It has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GBA2 antibody 17164-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GBA2 Polyclonal antibody (Cat# 17164-1-AP) has 1 total citation. It appears in Ecotoxicology and Environmental Safety (IF 6.6), published in 2026. The cited study investigates how PFOA exposure impairs sperm head shaping in mice, decreasing acrosome reaction and increasing DNA oxidative damage. The research field encompasses environmental toxicology and male reproductive biology. The antibody was used in Western blot (WB) with mouse samples.
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. |
