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  • KD/KO Validated

GBF1 Polyclonal antibody

GBF1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25183-1-AP

Synonyms

ARF1GEF, BFA resistant GEF 1, GBF1, KIAA0248



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25183-1-AP is GBF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBF1 fusion protein Ag16579
Full Name golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1
Calculated molecular weight 1859 aa, 206 kDa
Observed molecular weight 206 kDa
GenBank accession numberBC117681
Gene symbol GBF1
Gene ID (NCBI) 8729
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GBF1 antibody 25183-1-APDownload protocol
IHC protocol for GBF1 antibody 25183-1-APDownload protocol
IF protocol for GBF1 antibody 25183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.

Authors - Xiaobo Lei
  • KD Validated
humanWB

PLoS Pathog

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Authors - Tao Wang