Grancalcin Polyclonal antibody

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  WB analysis using 15787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15787-1-AP (Grancalcin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 15787-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15787-1-AP (Grancalcin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Grancalcin Polyclonal Antibody for WB, ELISA

Cat No. 15787-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GCA, GCL, Grancalcin

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Tested Applications

• Positive WB detected in: MCF-7 cells, HL-60 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15787-1-AP targets Grancalcin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8486

  Product name: Recombinant human GCA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-217 aa of BC005214

  Sequence: MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI

• Full Name: grancalcin, EF-hand calcium binding protein
• Calculated Molecular Weight: 217 aa, 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC005214
• Gene Symbol: Grancalcin
• Gene ID (NCBI): 25801
• RRID: AB_2878185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28676
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.