Tested Applications
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | human pancreas tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:40000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16317-1-AP targets GCAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9367 Product name: Recombinant human GCAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-419 aa of BC014457 Sequence: MWPGNAWRAALFWVPRGRRAQSALAQLRGILEGELEGICGAGTWKSERVITSRQGPHIRVDGVSGGILNFCANNYLGLSSHPEVIQAGLQALEEFGAGLSSVRFICGTQSIHKNLEAKIARFHQREDAILYPSCYDANAGLFEALLTPEDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLQEAQKHRLRLVATDGAFSMDGDIAPLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKMEAAGFTISGASHPICPVMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKAWIRVQISAVHSEEDIDRCVEAFVEVGRLHGALP Predict reactive species |
| Full Name | glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) |
| Calculated Molecular Weight | 419 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC014457 |
| Gene Symbol | GCAT |
| Gene ID (NCBI) | 23464 |
| RRID | AB_2263046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75600 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GCAT (Glycine acetyltransferase) is an enzyme involved in the metabolism of amino acids, specifically in the degradation of L-threonine to glycine. GCAT expression can be regulated epigenetically, and its downregulation has been linked to mitochondrial respiration defects, particularly in aging cells (PMID: 26000717).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GCAT antibody 16317-1-AP | Download protocol |
| WB protocol for GCAT antibody 16317-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GCAT Polyclonal antibody (Cat# 16317-1-AP) has 1 citation. It was cited in Life Science Alliance (2019), in a study on how the glycine cleavage system regulates pluripotent stem cell fate through senescence and epigenetic modifications. The research field encompasses stem cell biology, epigenetics, and cellular senescence.
| Species | Application | Title |
|---|---|---|
Life Sci Alliance Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications. |
Reviews
What customers say
One reviewer (Lana Yablonska) rated this product 4/5 stars in a Western Blot application using ST-Hdh mouse striatal cells. She used a 1:2000 dilution with overnight primary antibody incubation at 4°C, followed by an IRDye 800CW goat anti-rabbit secondary at 1:15000. The protocol involved mitochondrial fraction lysates run on a 4-12% Bis-Tris gradient gel and transferred overnight at 30V. The review included a representative SDS-PAGE image confirming successful detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lana (Verified Customer) (10-04-2019) | SDS-PAGE: 15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 cells. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4CSecondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000
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