GCAT Polyclonal antibody

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  WB analysis using 16317-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16317-1-AP (GCAT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 16317-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16317-1-AP (GCAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 16317-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16317-1-AP (GCAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16317-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16317-1-AP (GCAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16317-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16317-1-AP (GCAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GCAT Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16317-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, EC:2.3.1.29, Glycine acetyltransferase

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:40000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16317-1-AP targets GCAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9367

  Product name: Recombinant human GCAT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-419 aa of BC014457

  Sequence: MWPGNAWRAALFWVPRGRRAQSALAQLRGILEGELEGICGAGTWKSERVITSRQGPHIRVDGVSGGILNFCANNYLGLSSHPEVIQAGLQALEEFGAGLSSVRFICGTQSIHKNLEAKIARFHQREDAILYPSCYDANAGLFEALLTPEDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLQEAQKHRLRLVATDGAFSMDGDIAPLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKMEAAGFTISGASHPICPVMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKAWIRVQISAVHSEEDIDRCVEAFVEVGRLHGALP

• Full Name: glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase)
• Calculated Molecular Weight: 419 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC014457
• Gene Symbol: GCAT
• Gene ID (NCBI): 23464
• RRID: AB_2263046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75600
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCAT (Glycine acetyltransferase) is an enzyme involved in the metabolism of amino acids, specifically in the degradation of L-threonine to glycine. GCAT expression can be regulated epigenetically, and its downregulation has been linked to mitochondrial respiration defects, particularly in aging cells (PMID: 26000717).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Life Sci Alliance; Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.; Authors - Shengya Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lana (Verified Customer) (10-04-2019)

SDS-PAGE: 15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 cells. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4CSecondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: ST-Hdh mouse striatal cell line

GCAT Antibody Western Blot, validation (1:2000 dilution) in ST-Hdh mouse striatal cell line (Cat no:16317-1-AP)