Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human cervix tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18809-1-AP targets GCHFR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | GTP cyclohydrolase I feedback regulator |
| Calculated Molecular Weight | 10 kDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | BC112262 |
| Gene Symbol | GCHFR |
| Gene ID (NCBI) | 2644 |
| RRID | AB_10596912 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30047 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GCHFR,also named as p35 and GFRP, belongs to the GFRP family. GCHFR mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. It modulates BH4 synthesis and oxidative stress. This inhibition is reversed by L-phenylalanine.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GCHFR antibody 18809-1-AP | Download protocol |
| WB protocol for GCHFR antibody 18809-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GCHFR Polyclonal antibody (Cat# 18809-1-AP) has 1 citation. It appears in Antioxidants & Redox Signaling (2013), a study characterizing transgenic Gfrp knock-in mice and examining tetrahydrobiopterin's role in modulating normal tissue radiation responses. The research field encompasses radiation biology, oxidative stress, and BH4 metabolism. The antibody was used for WB and IHC in mouse samples.
| Species | Application | Title |
|---|---|---|
Antioxid Redox Signal Characterization of Transgenic Gfrp Knock-In Mice: Implications for Tetrahydrobiopterin in Modulation of Normal Tissue Radiation Responses.
|
