GCK Polyclonal antibody

• Featured Product
• KD/KO Validated

GCK Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15629-1-AP

Synonyms

HK4, HK IV, Hexokinase-D, Hexokinase-4, Glucokinase

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Validation Data Gallery

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  WB analysis using 15629-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15629-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15629-1-AP

  IP result of anti-GCK (IP:15629-1-AP, 4ug; Detection:15629-1-AP 1:500) with mouse liver tissue lysate 7000ug.

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  IHC staining of human liver cancer using 15629-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15629-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 15629-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 15629-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 15629-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15629-1-AP (GCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue, pig liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

This antibody is not recommended for WB detection of human samples.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

15629-1-AP targets GCK in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCK fusion protein Ag7904
• Full Name: glucokinase (hexokinase 4)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001890
• Gene Symbol: GCK
• Gene ID (NCBI): 2645
• RRID: AB_2878159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa. The westernblotting results of human cells we tested are not well, therefore, we do not recommend it to customers who would like to do WB involved in human.

Publications

Species	Application	Title
mouse	WB	J Diabetes Investig; Using recombinase-mediated cassette exchange to engineer MIN6 insulin-secreting cells based on a newly identified safe harbor locus.; Authors - Asami Furukawa ; KD Validated
mouse	WB	Mol Cell Endocrinol; Improved lipogenesis gene expression in liver is associated with elevated plasma angiotensin 1-7 after AT1 receptor blockade in insulin-resistant OLETF rats; Authors - Jose A Godoy-Lugo
mouse	WB,IHC	Biochem Pharmacol; Compromised chronic efficacy of a glucokinase activator AZD1656 in mouse models for common human GCKR variants; Authors - Brian E Ford