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GCK Polyclonal antibody

GCK Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


mouse, rat, human, pig





Cat no : 15629-1-AP


GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue, pig liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15629-1-AP targets GCK in WB, IP, IHC, ELISA applications and shows reactivity with mouse, rat, human, pig samples.

Tested Reactivity mouse, rat, human, pig
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCK fusion protein Ag7904
Full Name glucokinase (hexokinase 4)
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC001890
Gene Symbol GCK
Gene ID (NCBI) 2645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa. The westernblotting results of human cells we tested are not well, therefore, we do not recommend it to customers who would like to do WB involved in human.


Product Specific Protocols
WB protocol for GCK antibody 15629-1-APDownload protocol
IHC protocol for GCK antibody 15629-1-APDownload protocol
IP protocol for GCK antibody 15629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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