GCLM Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82623-1-RR (same clone as 82623-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82623-1-RR (same clone as 82623-1-PBS)

  WB result of GCLM antibody (82623-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 82623-1-RR (same clone as 82623-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82623-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82623-1-RR against Human GCLM were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

GCLM Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 82623-1-PBS

Clone No.4K16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

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Product Information

82623-1-PBS targets GCLM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5497

  Product name: Recombinant human GCLM protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-274 aa of BC041809

  Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

• Full Name: glutamate-cysteine ligase, modifier subunit
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC041809
• Gene Symbol: GCLM
• Gene ID (NCBI): 2730
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48507
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.