GCNA Polyclonal antibody

• 

  WB analysis using 25516-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 25516-1-AP (GCNA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

GCNA Polyclonal Antibody for WB, ELISA

Cat No. 25516-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

acidic repeat containing, ACRC, GCNA, NAAR1

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Tested Applications

• Positive WB detected in: human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25516-1-AP targets GCNA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21895

  Product name: Recombinant human ACRC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 343-691 aa of BC136259

  Sequence: NEGQIASDEEELVEAAAAVSQHDSSDDAGEQDLGENLSKPPSDPEANPEVSERKLPTEEEPAPVVEQSGKRKSKTKTIVEPPRKRQTKTKNIVEPPRKRQTKTKNIVEPLRKRKAKTKNVSVTPGHKKRGPSKKKPGAAKVEKRKTRTPKCKVPGCFLQDLEKSKKYSGKNLKRNKDELVQRIYDLFNRSVCDKKLPEKLRIGWNNKMVKTAGLCSTGEMWYPKWRRFAKIQIGLKVCDSADRIRDTLIHEMCHAASWLIDGIHDSHGDAWKYYARKSNRIHPELPRVTRCHNYKINYKVHYECTGCKTRIGCYTKSLDTSRFICAKCKGSLVMVPLTQKDGTRIVPHV

• Full Name: acidic repeat containing
• Calculated Molecular Weight: 691 aa, 76 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC136259
• Gene Symbol: GCNA
• Gene ID (NCBI): 93953
• RRID: AB_3085801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96QF7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCNA, also known as ACRC, belongs to the serine-aspartate repeat-containing protein (SDr) family. GCNA has remained tightly associated with sexual reproduction showing enriched expression within germ cells in both invertebrate and vertebrate species. GCNA has recently been implicated in resolving DPCs in germ cells and early embryos. GCNA is targeted to SUMOylated DPC proteins through its SUMO-interacting motifs (SIMs), reinforcing the importance of SUMOylation in DPC repair. The amino acid sequence homology of GCNA in humans and mice is very low. The calculated molecular weight of GCNA is 76 kDa. Sometimes higher molecular weight around 90 kDa can also be observed, which may be caused by SUMO modification (PMID: 31839537, 32329115).