GCNT1 Polyclonal antibody

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  WB analysis using 17845-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17845-1-AP (GCNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17845-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17845-1-AP (GCNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

GCNT1 Polyclonal Antibody for WB, ELISA

Cat No. 17845-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase, C2GlcNAcT, C2GNT1, C2GNT-L, Core 2 beta-1,6-N-acetylglucosaminyltransferase

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Tested Applications

• Positive WB detected in: TT cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17845-1-AP targets GCNT1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12264

  Product name: Recombinant human GCNT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 41-428 aa of BC074885

  Sequence: VSVRHLELAGENPSSDINCTKVLQGDVNEIQKVKLEILTVKFKKRPRWTPDDYINMTSDCSSFIKRRKYIVEPLSKEEAEFPIAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDTKSEDSYLAAVMGIASCFSNVFVASRLESVVYASWSRVQADLNCMKDLYAMSANWKYLINLCGMDFPIKTNLEIVRKLKLLMGENNLETERMPSHKEERWKKRYEVVNGKLTNTGTVKMLPPLETPLFSGSAYFVVSREYVGYVLQNEKIQKLMEWAQDTYSPDEYLWATIQRIPEVPGSLPASHKYDLSDMQAVARFVKWQYFEGDVSKGAPYPPCDGVHVRSVCIFGAGDLNWMLRKHHLFANKFDVDVDLFAIQCLDEHLRHKALETLKH

• Full Name: glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase)
• Calculated Molecular Weight: 428 aa, 50 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC074885
• Gene Symbol: GCNT1
• Gene ID (NCBI): 2650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02742
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucosaminyl (N-acetyl) transferase 1 (GCNT1, also known as C2GNT1), is a glycosyltransferase enzyme that plays an essential role in the formation of core 2 branched O-glycans that is crucial to the final definition of O-glycan structure (PMID: 37813880). GCNT1 promotes the growth of prostate tumors and regulates oncogenic gene expression pathways that are important for disease progression (PMID: 24854630).