GCNT2 Polyclonal antibody

GCNT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18118-1-AP

Synonyms

CCAT, GCNT2, GCNT2C, GCNT5, I branching enzyme, IGNT, II, NACGT1, NAGCT1, ULG3

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Validation Data Gallery

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  WB analysis of mouse kidney using 18118-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18118-1-AP (GCNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18118-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18118-1-AP (GCNT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 18118-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 18118-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 18118-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 18118-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18118-1-AP targets GCNT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCNT2 fusion protein Ag12635
• Full Name: glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
• Calculated Molecular Weight: 400 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC074801
• Gene Symbol: GCNT2
• Gene ID (NCBI): 2651
• RRID: AB_2232115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Glycobiology; MeCP2 regulated glycogenes contribute to proliferation and apoptosis of gastric cancer cells.; Authors - Yannan Qin